Method for preparative expression of genes in a cell-free system of conjugated transcription-translation

Vladimir I. Baranov (Inventor), Igor Morozov (Inventor), Alexandr S. Spirin (Inventor)

Research output: Patent

Abstract

The preparative expression of genes is effected in a cell-free system of conjugated transcription/translation comprising, as substrates, amino acids, ATP, GTP, CTP and UTP, the genes being used in the form of DNA molecules. As a result, products of gene expression are obtained in the system, including AMP, ADP, GDP, CDP, UDP, pyrophosphate and inorganic phosphate. During the functioning of the cell-free system of conjugated transcription/translation as the substrates are being used up with subsequent generation of the products, those products of gene expression which include polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphate and inorganic phosphate are extracted from the system. Simultaneously substrates are introduced into the system in the form of amino acids, ATP, GTP, CTP, UTP for maintaining their initial concentration.
Original languageEnglish
Patent numberWO9007003A1
IPCPCT/SU/00027
Priority date28/06/90
Filing date27/01/89
Publication statusPublished - 1990

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Cell-Free System
Cytidine Diphosphate
Cytidine Triphosphate
Uridine Triphosphate
Uridine Diphosphate
Adenosine Monophosphate
Guanosine Triphosphate
Gene Expression
Adenosine Diphosphate
Adenosine Triphosphate
Phosphates
Amino Acids
Peptides
DNA
Genes
diphosphoric acid

Cite this

Baranov, V. I., Morozov, I., & Spirin, A. S. (1990). IPC No. PCT/SU/00027. Method for preparative expression of genes in a cell-free system of conjugated transcription-translation. (Patent No. WO9007003A1).

Method for preparative expression of genes in a cell-free system of conjugated transcription-translation. / Baranov, Vladimir I. (Inventor); Morozov, Igor (Inventor); Spirin, Alexandr S. (Inventor).

IPC No.: PCT/SU/00027. Patent No.: WO9007003A1. Jan 27, 1989.

Research output: Patent

Baranov, VI, Morozov, I & Spirin, AS Jan. 27 1989, Method for preparative expression of genes in a cell-free system of conjugated transcription-translation, Patent No. WO9007003A1, IPC No. PCT/SU/00027.
Baranov, Vladimir I. (Inventor) ; Morozov, Igor (Inventor) ; Spirin, Alexandr S. (Inventor). / Method for preparative expression of genes in a cell-free system of conjugated transcription-translation. IPC No.: PCT/SU/00027. Patent No.: WO9007003A1. Jan 27, 1989.
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abstract = "The preparative expression of genes is effected in a cell-free system of conjugated transcription/translation comprising, as substrates, amino acids, ATP, GTP, CTP and UTP, the genes being used in the form of DNA molecules. As a result, products of gene expression are obtained in the system, including AMP, ADP, GDP, CDP, UDP, pyrophosphate and inorganic phosphate. During the functioning of the cell-free system of conjugated transcription/translation as the substrates are being used up with subsequent generation of the products, those products of gene expression which include polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphate and inorganic phosphate are extracted from the system. Simultaneously substrates are introduced into the system in the form of amino acids, ATP, GTP, CTP, UTP for maintaining their initial concentration.",
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N2 - The preparative expression of genes is effected in a cell-free system of conjugated transcription/translation comprising, as substrates, amino acids, ATP, GTP, CTP and UTP, the genes being used in the form of DNA molecules. As a result, products of gene expression are obtained in the system, including AMP, ADP, GDP, CDP, UDP, pyrophosphate and inorganic phosphate. During the functioning of the cell-free system of conjugated transcription/translation as the substrates are being used up with subsequent generation of the products, those products of gene expression which include polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphate and inorganic phosphate are extracted from the system. Simultaneously substrates are introduced into the system in the form of amino acids, ATP, GTP, CTP, UTP for maintaining their initial concentration.

AB - The preparative expression of genes is effected in a cell-free system of conjugated transcription/translation comprising, as substrates, amino acids, ATP, GTP, CTP and UTP, the genes being used in the form of DNA molecules. As a result, products of gene expression are obtained in the system, including AMP, ADP, GDP, CDP, UDP, pyrophosphate and inorganic phosphate. During the functioning of the cell-free system of conjugated transcription/translation as the substrates are being used up with subsequent generation of the products, those products of gene expression which include polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphate and inorganic phosphate are extracted from the system. Simultaneously substrates are introduced into the system in the form of amino acids, ATP, GTP, CTP, UTP for maintaining their initial concentration.

M3 - Patent

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ER -