TY - PAT
T1 - Method for preparative expression of genes in a cell-free system of conjugated transcription/translation
AU - Baranov, Vladimir I.
AU - Morozov, Igor
AU - Spirin, Alexandr S.
PY - 1989
Y1 - 1989
N2 - Prodn. of polypeptides is effected by expression of genes in the form of DNA mols. in a cell-free transcription/translation system contg. amino acids, ATP, GTP, CTP and UTP as substrates, resulting in the formation of prods. including polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphates and inorganic phosphates. The process is operated continuously, where the prods. are withdrawn as they are formed and the substrates are added so as to maintain their initial concns.
AB - Prodn. of polypeptides is effected by expression of genes in the form of DNA mols. in a cell-free transcription/translation system contg. amino acids, ATP, GTP, CTP and UTP as substrates, resulting in the formation of prods. including polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphates and inorganic phosphates. The process is operated continuously, where the prods. are withdrawn as they are formed and the substrates are added so as to maintain their initial concns.
UR - https://patentimages.storage.googleapis.com/d3/4f/5b/8c7dc34b2e3884/EP0401369A1.pdf
M3 - Patent
M1 - EP0401369A1
Y2 - 1989/01/27
ER -