Method for preparative expression of genes in a cell-free system of conjugated transcription/translation

Vladimir I. Baranov; Igor Morozov; Alexandr S. Spirin

Method for preparative expression of genes in a cell-free system of conjugated transcription/translation

Vladimir I. Baranov (Inventor), Igor Morozov (Inventor), Alexandr S. Spirin (Inventor)

Research output: Patent

Abstract

Prodn. of polypeptides is effected by expression of genes in the form of DNA mols. in a cell-free transcription/translation system contg. amino acids, ATP, GTP, CTP and UTP as substrates, resulting in the formation of prods. including polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphates and inorganic phosphates. The process is operated continuously, where the prods. are withdrawn as they are formed and the substrates are added so as to maintain their initial concns.

Original language	English
Patent number	EP0401369A1
Priority date	12/12/90
Filing date	27/01/89
Publication status	Published - 1989

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Baranov, V. I., Morozov, I., & Spirin, A. S. (1989). Method for preparative expression of genes in a cell-free system of conjugated transcription/translation. (Patent No. EP0401369A1).

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abstract = "Prodn. of polypeptides is effected by expression of genes in the form of DNA mols. in a cell-free transcription/translation system contg. amino acids, ATP, GTP, CTP and UTP as substrates, resulting in the formation of prods. including polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphates and inorganic phosphates. The process is operated continuously, where the prods. are withdrawn as they are formed and the substrates are added so as to maintain their initial concns.",

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