Method for preparative expression of genes in a cell-free system of conjugated transcription/translation

Vladimir I. Baranov; Igor Morozov; Alexandr S. Spirin

Method for preparative expression of genes in a cell-free system of conjugated transcription/translation

Vladimir I. Baranov (Inventor), Igor Morozov (Inventor), Alexandr S. Spirin (Inventor)

School of Life Sciences

Research output: Patent

Abstract

Prodn. of polypeptides is effected by expression of genes in the form of DNA mols. in a cell-free transcription/translation system contg. amino acids, ATP, GTP, CTP and UTP as substrates, resulting in the formation of prods. including polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphates and inorganic phosphates. The process is operated continuously, where the prods. are withdrawn as they are formed and the substrates are added so as to maintain their initial concns.

Original language	English
Patent number	EP0401369A1
IPC	C12P 21/00,C12N 15/00
Priority date	22/12/88
Filing date	27/01/89
Publication status	Published - 12 Dec 1990

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1 Patent

Method for preparative expression of genes in a cell-free system of conjugated transcription-translation
Baranov, V. I., Morozov, I. & Spirin, A. S., 28 Jun 1990, IPC No. C12N 15/00, C12P 21/00, Patent No. WO9007003A1, 27 Jan 1989, Priority date 22 Dec 1988, Priority No. SU4618624/31
Research output: Patent

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title = "Method for preparative expression of genes in a cell-free system of conjugated transcription/translation",

abstract = "Prodn. of polypeptides is effected by expression of genes in the form of DNA mols. in a cell-free transcription/translation system contg. amino acids, ATP, GTP, CTP and UTP as substrates, resulting in the formation of prods. including polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphates and inorganic phosphates. The process is operated continuously, where the prods. are withdrawn as they are formed and the substrates are added so as to maintain their initial concns.",

author = "Baranov, {Vladimir I.} and Igor Morozov and Spirin, {Alexandr S.}",

year = "1990",

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TY - PAT

T1 - Method for preparative expression of genes in a cell-free system of conjugated transcription/translation

AU - Baranov, Vladimir I.

AU - Morozov, Igor

AU - Spirin, Alexandr S.

PY - 1990/12/12

Y1 - 1990/12/12

N2 - Prodn. of polypeptides is effected by expression of genes in the form of DNA mols. in a cell-free transcription/translation system contg. amino acids, ATP, GTP, CTP and UTP as substrates, resulting in the formation of prods. including polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphates and inorganic phosphates. The process is operated continuously, where the prods. are withdrawn as they are formed and the substrates are added so as to maintain their initial concns.

AB - Prodn. of polypeptides is effected by expression of genes in the form of DNA mols. in a cell-free transcription/translation system contg. amino acids, ATP, GTP, CTP and UTP as substrates, resulting in the formation of prods. including polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphates and inorganic phosphates. The process is operated continuously, where the prods. are withdrawn as they are formed and the substrates are added so as to maintain their initial concns.

M3 - Patent

M1 - EP0401369A1

Y2 - 1989/01/27

ER -

Method for preparative expression of genes in a cell-free system of conjugated transcription/translation

Abstract

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Research output

Method for preparative expression of genes in a cell-free system of conjugated transcription-translation

Cite this