Macrophages in gene therapy: cellular delivery vehicles and in vivo targets

B Burke, S Sumner, N Maitland, C E Lewis

Research output: Contribution to journalReview article

102 Citations (Scopus)

Abstract

The appearance and activation of macrophages are thought to be rapid events in the development of many pathological lesions, including malignant tumors, atherosclerotic plaques, and arthritic joints. This has prompted recent attempts to use macrophages as novel cellular vehicles for gene therapy, in which macrophages are genetically modified ex vivo and then reintroduced into the body with the hope that a proportion will then home to the diseased site. Here, we critically review the efficacy of various gene transfer methods (viral, bacterial, protozoan, and various chemical and physical methods) in transfecting macrophages in vitro, and the results obtained when transfected macrophages are used as gene delivery vehicles. Finally, we discuss the use of various viral and nonviral methods to transfer genes to macrophages in vivo. As will be seen, definitive evidence for the use of macrophages as gene transfer vehicles has yet to be provided and awaits detailed trafficking studies in vivo. Moreover, although methods for transfecting macrophages have improved considerably in efficiency in recent years, targeting of gene transfer specifically to macrophages in vivo remains a problem. However, possible solutions to this include placing transgenes under the control of macrophage-specific promoters to limit expression to macrophages or stably transfecting CD34(+) precursors of monocytes/macrophages and then differentiating these cells into monocytes/macrophages ex vivo. The latter approach could conceivably lead to the bone marrow precursor cells of patients with inherited genetic disorders being permanently fortified or even replaced with genetically modified cells.

Original languageEnglish
Pages (from-to)417-28
Number of pages12
JournalJournal of Leukocyte Biology
Volume72
Issue number3
Publication statusPublished - Sep 2002
Externally publishedYes

Fingerprint

Genetic Therapy
Macrophages
Genes
Monocytes
Inborn Genetic Diseases
Gene Targeting
Macrophage Activation
Atherosclerotic Plaques
Transgenes
Bone Marrow Cells
Arthritis
Joints

Keywords

  • Adenoviridae/genetics
  • Animals
  • Arthritis/pathology
  • Cell Movement
  • Cytokines/physiology
  • DNA/genetics
  • Genetic Therapy/methods
  • Genetic Vectors/genetics
  • Growth Substances/physiology
  • HIV Infections/therapy
  • Humans
  • Immunotherapy, Adoptive/methods
  • Leishmania/genetics
  • Listeria/genetics
  • Lysosomal Storage Diseases/therapy
  • Macrophages/microbiology
  • Mice
  • Neoplasms/pathology
  • Rats
  • Retroviridae/genetics
  • Salmonella/genetics
  • Transfection/methods
  • Transformation, Genetic

Cite this

Macrophages in gene therapy : cellular delivery vehicles and in vivo targets. / Burke, B; Sumner, S; Maitland, N; Lewis, C E.

In: Journal of Leukocyte Biology, Vol. 72, No. 3, 09.2002, p. 417-28.

Research output: Contribution to journalReview article

Burke, B, Sumner, S, Maitland, N & Lewis, CE 2002, 'Macrophages in gene therapy: cellular delivery vehicles and in vivo targets' Journal of Leukocyte Biology, vol. 72, no. 3, pp. 417-28.
Burke, B ; Sumner, S ; Maitland, N ; Lewis, C E. / Macrophages in gene therapy : cellular delivery vehicles and in vivo targets. In: Journal of Leukocyte Biology. 2002 ; Vol. 72, No. 3. pp. 417-28.
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AB - The appearance and activation of macrophages are thought to be rapid events in the development of many pathological lesions, including malignant tumors, atherosclerotic plaques, and arthritic joints. This has prompted recent attempts to use macrophages as novel cellular vehicles for gene therapy, in which macrophages are genetically modified ex vivo and then reintroduced into the body with the hope that a proportion will then home to the diseased site. Here, we critically review the efficacy of various gene transfer methods (viral, bacterial, protozoan, and various chemical and physical methods) in transfecting macrophages in vitro, and the results obtained when transfected macrophages are used as gene delivery vehicles. Finally, we discuss the use of various viral and nonviral methods to transfer genes to macrophages in vivo. As will be seen, definitive evidence for the use of macrophages as gene transfer vehicles has yet to be provided and awaits detailed trafficking studies in vivo. Moreover, although methods for transfecting macrophages have improved considerably in efficiency in recent years, targeting of gene transfer specifically to macrophages in vivo remains a problem. However, possible solutions to this include placing transgenes under the control of macrophage-specific promoters to limit expression to macrophages or stably transfecting CD34(+) precursors of monocytes/macrophages and then differentiating these cells into monocytes/macrophages ex vivo. The latter approach could conceivably lead to the bone marrow precursor cells of patients with inherited genetic disorders being permanently fortified or even replaced with genetically modified cells.

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