Abstract
The effects of adipose-derived mesenchymal stem cells (ADMSC) transplantation on degeneration, regeneration and skeletal muscle function were investigated in dystrophin-deficient mice (24-week-old). ADMSC transplantation improved muscle strength and, resistance to fatigue. An increase in fiber cross-sectional area and in the number of fibers with centralized nuclei and augment of myogenin content were observed. In ADMSC-treated muscles a decrease in muscle content of TNF-α, IL-6 and oxidative stress measured by Amplex(®) reagent were observed. The level of TGF-β1 was lowered whereas that of VEGF, IL-10 and IL-4 were increased by ADMSC treatment. An increase in markers of macrophage M1 (CD11 and F4-80) and a decrease in T lymphocyte marker (CD3) and arginase-1 were also observed in ADMSCs-treated dystrophic muscle. No change was observed in iNOS expression. Increased phosphorylation of Akt, p70S6k and 4E-BP1 was found in dystrophic muscles treated with ADMSC. These results suggest that ADMSC transplantation modulates inflammation and improves muscle tissue regeneration, ameliorating the dystrophic phenotype in dystrophin-deficient mice.
Original language | English |
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Pages (from-to) | 363-374 |
Number of pages | 12 |
Journal | Stem Cell Reviews and Reports |
Volume | 8 |
Early online date | 27 Aug 2011 |
DOIs | |
Publication status | Published - Jun 2012 |
Externally published | Yes |
Keywords
- Adipose Tissue/cytology
- Biomarkers/metabolism
- Cytokines/metabolism
- Dystrophin/deficiency
- Inflammation/pathology
- Inflammation Mediators/metabolism
- Macrophages/metabolism
- Mesenchymal Stem Cell Transplantation
- Mesenchymal Stem Cells/cytology
- Mice
- Muscle, Skeletal/pathology
- Muscular Dystrophy, Animal/pathology
- Myogenin/metabolism
- Neovascularization, Physiologic