Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response

D. G. Waugh, J. Lawrence, P. Shukla, C. Chan, I. Hussain, H. C. Man, Glynn C. Smith

Research output: Chapter in Book/Report/Conference proceedingConference proceeding

Abstract

Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.

Original languageEnglish
Title of host publicationIndustrial Laser Applications Symposium, ILAS 2015
PublisherSPIE
Volume9657
ISBN (Electronic)9781628418705
DOIs
Publication statusPublished - 1 Jul 2015
Externally publishedYes
Event4th Industrial Laser Applications Symposium - Kenilworth, United Kingdom
Duration: 17 Mar 201518 Mar 2015

Conference

Conference4th Industrial Laser Applications Symposium
Abbreviated titleILAS 2015
CountryUnited Kingdom
CityKenilworth
Period17/03/1518/03/15

Keywords

  • cell viability
  • CO laser
  • fiber laser
  • laser surface treatment
  • MTT
  • stem cells

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Waugh, D. G., Lawrence, J., Shukla, P., Chan, C., Hussain, I., Man, H. C., & Smith, G. C. (2015). Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response. In Industrial Laser Applications Symposium, ILAS 2015 (Vol. 9657). [96570L] SPIE. https://doi.org/10.1117/12.2182432