Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy

O. Sparagano, G. R. Loria, M. J. Gubbels, A. P. De Vos, S. Caracappa, F. Jongejan

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8% and 23.5% in April and November, respectively.

Original languageEnglish
Pages (from-to)533-539
Number of pages7
JournalAnnals of the New York Academy of Sciences
Publication statusPublished - 1 Jan 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science


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