Initiation of Bone Regeneration in Adult Baboons by Osteogenin, a Bone Morphogenetic Protein

U Ripamonti, S Ma, NS Cunningham, L Yeates, AH Reddi

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Osteogenin, and related bone morphogenetic proteins, induce endochondral bone differentiation through a cascade of events which include formation of cartilage, hypertrophy and calcification of the cartilage, vascular invasion, differentiation of osteoblasts, and formation of bone. These events have been studied in a postnatal model of bone development in rodents. Information concerning the morphogenetic potential of osteogenin in primates is a prerequisite for potential clinical application in man. The efficacy of allogeneic osteogenin in primates was investigated in both extraskeletal and skeletal sites in 19-Chacma baboons (Papio ursinus). Osteogenin was isolated from demineralized baboon bone matrix and purified by chromatography on heparin-Sepharose, hydroxyapatite, and Sephacryl S-200. Protein fractions with a molecular mass range of 26-42 kDa induced cartilage and bone differentiation in the subcutaneous space of rats. Final purification to homogeneity was obtained by electroendosmotic elution from a preparative sodium dodecyl sulphate (SDS) polyacrylamide gel, resulting in a single band on a SDS-polyacrylamide gel with an apparent molecular mass of 30-34 kDa, with biological activity in rats. The osteoinductive potential of osteogenin in primates was tested first in intramuscular sites in baboons and found to be active. The bone regeneration potential was investigated in nonhealing calvarial defects surgically prepared in adult male baboons. Baboon osteogenin induced complete regeneration of the cranial wound. These findings in adult primates establish a primary role for osteogenin in initiation and promotion of osteogenesis; and imply a potential therapeutic application based on cell biology of extracellular matrix-cell interactions.
Original languageEnglish
Pages (from-to)369-380
Number of pages12
JournalMatrix
Volume12
Issue number5
DOIs
Publication statusPublished - Nov 1992
Externally publishedYes

Fingerprint

Bone Morphogenetic Protein 3
Bone Morphogenetic Proteins
Bone Regeneration
Papio
Primates
Papio ursinus
Cartilage
Osteogenesis
Sodium Dodecyl Sulfate
Vascular Calcification
Bone and Bones
Bone Matrix
Bone Development
Durapatite
Osteoblasts
Cell Communication
Hypertrophy
Extracellular Matrix
Cell Biology
Chromatography

Keywords

  • bone induction
  • bone morphogenetic proteins
  • collagenous matrix
  • osteogenin
  • primates

Cite this

Initiation of Bone Regeneration in Adult Baboons by Osteogenin, a Bone Morphogenetic Protein. / Ripamonti, U; Ma, S; Cunningham, NS; Yeates, L; Reddi, AH.

In: Matrix, Vol. 12, No. 5, 11.1992, p. 369-380.

Research output: Contribution to journalArticle

Ripamonti, U ; Ma, S ; Cunningham, NS ; Yeates, L ; Reddi, AH. / Initiation of Bone Regeneration in Adult Baboons by Osteogenin, a Bone Morphogenetic Protein. In: Matrix. 1992 ; Vol. 12, No. 5. pp. 369-380.
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