Inhibition of T cell response in vitro by an antiody against a novel lymphocyte surface molecule (QCA-1)

Jaap Kamplnga; Frans G.M. Kroese; Gerda H. Pol; Boelo Meedendrop; Jaap Van Eendenburg; Herman Groen; Johan Van Den Bogaerde; Paul Nieuwenhuis; Bruce Roser; Richard Aspinall

doi:10.1093/intimm/2.10.915

Inhibition of T cell response in vitro by an antiody against a novel lymphocyte surface molecule (QCA-1)

Jaap Kamplnga
, Frans G.M. Kroese
, Gerda H. Pol
, Boelo Meedendrop
, Jaap Van Eendenburg
, Herman Groen
, Johan Van Den Bogaerde
, Paul Nieuwenhuis
, Bruce Roser
, Richard Aspinall

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We have identified an antigen present on the surface of lymphocytes in the rat which appears to play an important role in the preliminary stages of the immune response. This antigen, which we have called quiescent cell antigen 1 because of its apparent expression only on quiescent cells, is present on the majority of peripheral T and B cells and a small percentage of thymocytes which are located mainly in the medullary region. SDS -PAGE analysis of membrane molecules shows two bands on unreduced gels at -43 and 47 kd. On reduction the bands ran at -46 and 60 kd. When a monoclonal antibody against this antigen (HIS45) is present in an allogeneic mixed leukocyte reaction, it inhibtts the proliferation of responding cells completely. When the antibody HIS45 is added to cytotoxic T lymphocyte mediated iysis assays it does not inhibit lysls nor does it affect the spectficity of this lysls. Comparison with other antibodies which have been reported to affect lymphocyte function in rats and in other species fail to reveal any which have similar properties.

Original language	English
Pages (from-to)	915-920
Number of pages	6
Journal	International Immunology
Volume	2
Issue number	10
DOIs	https://doi.org/10.1093/intimm/2.10.915
Publication status	Published - 1 Oct 1990
Externally published	Yes

Funding

The authors gratefully acknowledge A. F. Williams, C Dijkstra, and T. A. Springer for kindly providing antibodies, F Klatter and S Colaco for expert technical assistance, and P. van der Syde for expert photography. This work was supported by a grant from the Dutch Foundation for Medical and Hearth Research, MEDIGON, which is subsidized by the Netherlands Organization for Advancement of Pure Research (ZWO, Grant 900-505-201), the Quadrant Research Foundation, The Royal Society of Medicine, and the Medical Research Council of the United Kingdom.

Keywords

Antigen recognition
QCA-1
Rat
T cell activation

ASJC Scopus subject areas

Immunology

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10.1093/intimm/2.10.915

Cite this

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title = "Inhibition of T cell response in vitro by an antiody against a novel lymphocyte surface molecule (QCA-1)",

abstract = "We have identified an antigen present on the surface of lymphocytes in the rat which appears to play an important role in the preliminary stages of the immune response. This antigen, which we have called quiescent cell antigen 1 because of its apparent expression only on quiescent cells, is present on the majority of peripheral T and B cells and a small percentage of thymocytes which are located mainly in the medullary region. SDS -PAGE analysis of membrane molecules shows two bands on unreduced gels at -43 and 47 kd. On reduction the bands ran at -46 and 60 kd. When a monoclonal antibody against this antigen (HIS45) is present in an allogeneic mixed leukocyte reaction, it inhibtts the proliferation of responding cells completely. When the antibody HIS45 is added to cytotoxic T lymphocyte mediated iysis assays it does not inhibit lysls nor does it affect the spectficity of this lysls. Comparison with other antibodies which have been reported to affect lymphocyte function in rats and in other species fail to reveal any which have similar properties.",

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author = "Jaap Kamplnga and Kroese, \{Frans G.M.\} and Pol, \{Gerda H.\} and Boelo Meedendrop and Eendenburg, \{Jaap Van\} and Herman Groen and Bogaerde, \{Johan Van Den\} and Paul Nieuwenhuis and Bruce Roser and Richard Aspinall",

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doi = "10.1093/intimm/2.10.915",

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AU - Kamplnga, Jaap

AU - Kroese, Frans G.M.

AU - Pol, Gerda H.

AU - Meedendrop, Boelo

AU - Eendenburg, Jaap Van

AU - Groen, Herman

AU - Bogaerde, Johan Van Den

AU - Nieuwenhuis, Paul

AU - Roser, Bruce

AU - Aspinall, Richard

PY - 1990/10/1

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N2 - We have identified an antigen present on the surface of lymphocytes in the rat which appears to play an important role in the preliminary stages of the immune response. This antigen, which we have called quiescent cell antigen 1 because of its apparent expression only on quiescent cells, is present on the majority of peripheral T and B cells and a small percentage of thymocytes which are located mainly in the medullary region. SDS -PAGE analysis of membrane molecules shows two bands on unreduced gels at -43 and 47 kd. On reduction the bands ran at -46 and 60 kd. When a monoclonal antibody against this antigen (HIS45) is present in an allogeneic mixed leukocyte reaction, it inhibtts the proliferation of responding cells completely. When the antibody HIS45 is added to cytotoxic T lymphocyte mediated iysis assays it does not inhibit lysls nor does it affect the spectficity of this lysls. Comparison with other antibodies which have been reported to affect lymphocyte function in rats and in other species fail to reveal any which have similar properties.

AB - We have identified an antigen present on the surface of lymphocytes in the rat which appears to play an important role in the preliminary stages of the immune response. This antigen, which we have called quiescent cell antigen 1 because of its apparent expression only on quiescent cells, is present on the majority of peripheral T and B cells and a small percentage of thymocytes which are located mainly in the medullary region. SDS -PAGE analysis of membrane molecules shows two bands on unreduced gels at -43 and 47 kd. On reduction the bands ran at -46 and 60 kd. When a monoclonal antibody against this antigen (HIS45) is present in an allogeneic mixed leukocyte reaction, it inhibtts the proliferation of responding cells completely. When the antibody HIS45 is added to cytotoxic T lymphocyte mediated iysis assays it does not inhibit lysls nor does it affect the spectficity of this lysls. Comparison with other antibodies which have been reported to affect lymphocyte function in rats and in other species fail to reveal any which have similar properties.

KW - Antigen recognition

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KW - T cell activation

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ER -

Inhibition of T cell response in vitro by an antiody against a novel lymphocyte surface molecule (QCA-1)

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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