Ingestion of lean meat elevates muscle inositol hexakisphosphate kinase 1 protein content independent of a distinct post-prandial circulating proteome in young adults with obesity

Richie Barclay, Joseph W. Beals, Jenny Drnevich, Brian S. Imai, Peter M. Yau, Alexander V. Ulanov, Neale A. Tillin, Martha Villegas-Montes, Scott A. Paluska, Peter W. Watt, Michael De Lisio, Nicholas A. Burd, Richard Mackenzie

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Background: We have recently shown that a novel signalling kinase, inositol hexakisphosphate kinase 1 (IP6K1), is implicated in whole-body insulin resistance via its inhibitory action on Akt. Insulin and insulin like growth factor 1 (IGF-1) share many intracellular processes with both known to play a key role in glucose and protein metabolism in skeletal muscle. Aims: We aimed to compare IGF/IP6K1/Akt signalling and the plasma proteomic signature in individuals with a range of BMIs after ingestion of lean meat. Methods: Ten lean [Body mass index (BMI) (in kg/m 2): 22.7 ± 0.4; Homeostatic model assessment of insulin resistance (HOMA IR): 1.36 ± 0.17], 10 overweight (BMI: 27.1 ± 0.5; HOMA IR: 1.25 ± 0.11), and 10 obese (BMI: 35.9 ± 1.3; HOMA IR: 5.82 ± 0.81) adults received primed continuous L-[ring- 13C 6]phenylalanine infusions. Blood and muscle biopsy samples were collected at 0 min (post-absorptive), 120 min and 300 min relative to the ingestion of 170 g pork loin (36 g protein and 5 g fat) to examine skeletal muscle protein signalling, plasma proteomic signatures, and whole-body phenylalanine disappearance rates (R d). Results: Phenylalanine R d was not different in obese compared to lean individuals at all time points and was not responsive to a pork ingestion (basal, P = 0.056; 120 & 300 min, P > 0.05). IP6K1 was elevated in obese individuals at 120 min post-prandial vs basal (P < 0.05). There were no acute differences plasma proteomic profiles between groups in the post-prandial state (P > 0.05). Conclusions: These data demonstrate, for the first time that muscle IP6K1 protein content is elevated after lean meat ingestion in obese adults, suggesting that IP6K1 may be contributing to the dysregulation of nutrient uptake in skeletal muscle. In addition, proteomic analysis showed no differences in proteomic signatures between obese, overweight or lean individuals.

Original languageEnglish
Article number153996
Number of pages11
JournalMetabolism: Clinical and Experimental
Volume102
Early online date31 Oct 2019
DOIs
Publication statusPublished - Jan 2020
Externally publishedYes

Keywords

  • Insulin resistance
  • Obesity
  • Amino acids
  • Anabolic resistance
  • IP6K1

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