Induction of long-term survival of hamster heart xenografts in rats

Johan Van Den Bogaerde, Richard Aspinall, Ming Wei Wang, Nat Cary, Sue Lim, Les Wright, David White

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Abstract

The aim of this study was to determine the mechanisms responsible for concordant xenograft rejection using the hamster-to-rat heart graft model. Even though it was known that rat CD4 positive T cells proliferated to hamster stimulators in mixed lymphocyte reactions, the depletion of CD4 positive T cells in rat recipients did not lead to an extension of xenograft survival. Suppression of T cell immunity using other monoclonal antibodies or cyclosporine also failed to improve survival. Only by depleting complement with cobra-venom factor could hamster xenograft survival be prolonged, and long-term survival was achieved by combining CsA with COF. High-antibody titers to hamster cells were found after transplantation of hamster hearts, and evidence is presented that rejection of these “concordant” xenografts is mediated primarily by antibody-complement mechanisms. The antihamster antibodies were produced in the absence of T cell help, which suggests that antibodymediated graft destruction cannot be inhibited by suppression or depletion of T cells. Pharmacologic depletion of complement for the clinical application of concordant xenografts is a promising avenue of future research.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalTransplantation
Volume52
Issue number1
Publication statusPublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Transplantation

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  • Cite this

    Van Den Bogaerde, J., Aspinall, R., Wang, M. W., Cary, N., Lim, S., Wright, L., & White, D. (1991). Induction of long-term survival of hamster heart xenografts in rats. Transplantation, 52(1), 15-20.