Induction of long-term survival of hamster heart xenografts in rats

Johan Van Den Bogaerde, Richard Aspinall, Ming Wei Wang, Nat Cary, Sue Lim, Les Wright, David White

Research output: Contribution to journalArticle

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Abstract

The aim of this study was to determine the mechanisms responsible for concordant xenograft rejection using the hamster-to-rat heart graft model. Even though it was known that rat CD4 positive T cells proliferated to hamster stimulators in mixed lymphocyte reactions, the depletion of CD4 positive T cells in rat recipients did not lead to an extension of xenograft survival. Suppression of T cell immunity using other monoclonal antibodies or cyclosporine also failed to improve survival. Only by depleting complement with cobra-venom factor could hamster xenograft survival be prolonged, and long-term survival was achieved by combining CsA with COF. High-antibody titers to hamster cells were found after transplantation of hamster hearts, and evidence is presented that rejection of these “concordant” xenografts is mediated primarily by antibody-complement mechanisms. The antihamster antibodies were produced in the absence of T cell help, which suggests that antibodymediated graft destruction cannot be inhibited by suppression or depletion of T cells. Pharmacologic depletion of complement for the clinical application of concordant xenografts is a promising avenue of future research.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalTransplantation
Volume52
Issue number1
Publication statusPublished - 1991
Externally publishedYes

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Heterografts
Cricetinae
T-Lymphocytes
Antibodies
Lymphocyte Depletion
Transplants
Mixed Lymphocyte Culture Test
Heart Transplantation
Cyclosporine
Immunity
Monoclonal Antibodies

ASJC Scopus subject areas

  • Transplantation

Cite this

Van Den Bogaerde, J., Aspinall, R., Wang, M. W., Cary, N., Lim, S., Wright, L., & White, D. (1991). Induction of long-term survival of hamster heart xenografts in rats. Transplantation, 52(1), 15-20.

Induction of long-term survival of hamster heart xenografts in rats. / Van Den Bogaerde, Johan; Aspinall, Richard; Wang, Ming Wei; Cary, Nat; Lim, Sue; Wright, Les; White, David.

In: Transplantation, Vol. 52, No. 1, 1991, p. 15-20.

Research output: Contribution to journalArticle

Van Den Bogaerde, J, Aspinall, R, Wang, MW, Cary, N, Lim, S, Wright, L & White, D 1991, 'Induction of long-term survival of hamster heart xenografts in rats' Transplantation, vol. 52, no. 1, pp. 15-20.
Van Den Bogaerde J, Aspinall R, Wang MW, Cary N, Lim S, Wright L et al. Induction of long-term survival of hamster heart xenografts in rats. Transplantation. 1991;52(1):15-20.
Van Den Bogaerde, Johan ; Aspinall, Richard ; Wang, Ming Wei ; Cary, Nat ; Lim, Sue ; Wright, Les ; White, David. / Induction of long-term survival of hamster heart xenografts in rats. In: Transplantation. 1991 ; Vol. 52, No. 1. pp. 15-20.
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