Increased yields of IL-2 in media conditioned by MLA 144 cells

R. Aspinall; A. O'Gorman

doi:10.1016/0022-1759(87)90219-5

Increased yields of IL-2 in media conditioned by MLA 144 cells

R. Aspinall
, A. O'Gorman

University of Oxford

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The gibbon leukamia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 × 10⁵ cells/ml in DMEM containing 10% FBS and supplemented with 2-ME at 5 × 10^-5 M and PMA at 10 ng/ml.

Original language	English
Pages (from-to)	79-84
Number of pages	6
Journal	Journal of Immunological Methods
Volume	101
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(87)90219-5
Publication status	Published - 16 Jun 1987
Externally published	Yes

Funding

This work was supported by the Cancer Research Campaign.

Keywords

Interleukin-2
MLA 144 cell line
Phorbol myristate acetate

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(87)90219-5

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abstract = "The gibbon leukamia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 × 105 cells/ml in DMEM containing 10\% FBS and supplemented with 2-ME at 5 × 10-5 M and PMA at 10 ng/ml.",

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AU - O'Gorman, A.

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N2 - The gibbon leukamia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 × 105 cells/ml in DMEM containing 10% FBS and supplemented with 2-ME at 5 × 10-5 M and PMA at 10 ng/ml.

AB - The gibbon leukamia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 × 105 cells/ml in DMEM containing 10% FBS and supplemented with 2-ME at 5 × 10-5 M and PMA at 10 ng/ml.

KW - Interleukin-2

KW - MLA 144 cell line

KW - Phorbol myristate acetate

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Increased yields of IL-2 in media conditioned by MLA 144 cells

Abstract

Funding

Keywords

ASJC Scopus subject areas

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