Increased yields of IL-2 in media conditioned by MLA 144 cells

R. Aspinall, A. O'Gorman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The gibbon leukamia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 × 105 cells/ml in DMEM containing 10% FBS and supplemented with 2-ME at 5 × 10-5 M and PMA at 10 ng/ml.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalJournal of Immunological Methods
Volume101
Issue number1
DOIs
Publication statusPublished - 16 Jun 1987
Externally publishedYes

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Keywords

  • Interleukin-2
  • MLA 144 cell line
  • Phorbol myristate acetate

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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