Increased yields of IL-2 in media conditioned by MLA 144 cells

R. Aspinall, A. O'Gorman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The gibbon leukamia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 × 105 cells/ml in DMEM containing 10% FBS and supplemented with 2-ME at 5 × 10-5 M and PMA at 10 ng/ml.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalJournal of Immunological Methods
Volume101
Issue number1
DOIs
Publication statusPublished - 16 Jun 1987
Externally publishedYes

Fingerprint

Conditioned Culture Medium
Interleukin-2
Cell Line
Hylobates
Mercaptoethanol
Growth
T-Lymphocytes

Keywords

  • Interleukin-2
  • MLA 144 cell line
  • Phorbol myristate acetate

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Increased yields of IL-2 in media conditioned by MLA 144 cells. / Aspinall, R.; O'Gorman, A.

In: Journal of Immunological Methods, Vol. 101, No. 1, 16.06.1987, p. 79-84.

Research output: Contribution to journalArticle

@article{5542a254867e46a09e25b1f385fe8b5c,
title = "Increased yields of IL-2 in media conditioned by MLA 144 cells",
abstract = "The gibbon leukamia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 × 105 cells/ml in DMEM containing 10{\%} FBS and supplemented with 2-ME at 5 × 10-5 M and PMA at 10 ng/ml.",
keywords = "Interleukin-2, MLA 144 cell line, Phorbol myristate acetate",
author = "R. Aspinall and A. O'Gorman",
year = "1987",
month = "6",
day = "16",
doi = "10.1016/0022-1759(87)90219-5",
language = "English",
volume = "101",
pages = "79--84",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Increased yields of IL-2 in media conditioned by MLA 144 cells

AU - Aspinall, R.

AU - O'Gorman, A.

PY - 1987/6/16

Y1 - 1987/6/16

N2 - The gibbon leukamia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 × 105 cells/ml in DMEM containing 10% FBS and supplemented with 2-ME at 5 × 10-5 M and PMA at 10 ng/ml.

AB - The gibbon leukamia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 × 105 cells/ml in DMEM containing 10% FBS and supplemented with 2-ME at 5 × 10-5 M and PMA at 10 ng/ml.

KW - Interleukin-2

KW - MLA 144 cell line

KW - Phorbol myristate acetate

UR - http://www.scopus.com/inward/record.url?scp=0023197991&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(87)90219-5

DO - 10.1016/0022-1759(87)90219-5

M3 - Article

VL - 101

SP - 79

EP - 84

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1

ER -