In vitro mesenchymal stem cell response to a CO2 laser modified polymeric material

D. G. Waugh, I. Hussain, J. Lawrence, G. C. Smith, D. Cosgrove, C. Toccaceli

Research output: Contribution to journalArticle

5 Citations (Scopus)
50 Downloads (Pure)

Abstract

With an ageing world population it is becoming significantly apparent that there is a need to produce implants and platforms to manipulate stem cell growth on a pharmaceutical scale. This is needed to meet the socio-economic demands of many countries worldwide. This paper details one of the first ever studies in to the manipulation of stem cell growth on CO2 laser surface treated nylon 6,6 highlighting its potential as an inexpensive platform to manipulate stem cell growth on a pharmaceutical scale. Through CO2 laser surface treatment discrete changes to the surfaces were made. That is, the surface roughness of the nylon 6,6 was increased by up to 4.3 μm, the contact angle was modulated by up to 5° and the surface oxygen content increased by up to 1 atom %. Following mesenchymal stem cell growth on the laser treated samples, it was identified that CO2 laser surface treatment gave rise to an enhanced response with an increase in viable cell count of up to 60,000 cells/ml when compared to the as-received sample. The effect of surface parameters modified by the CO2 laser surface treatment on the mesenchymal stem cell response is also discussed along with potential trends that could be identified to govern the mesenchymal stem cell response.

Original languageEnglish
Pages (from-to)727-736
Number of pages10
JournalMaterials Science and Engineering C
Volume67
Early online date16 May 2016
DOIs
Publication statusPublished - 1 Oct 2016
Externally publishedYes

Bibliographical note

Publisher Statement: NOTICE: this is the author’s version of a work that was accepted for publication in Materials Science and Engineering C. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Science and Engineering C, [67 (2017)]DOI: 10.1016/j.msec.2016.05.055

http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

  • Adhesion
  • CO laser
  • Contact angle
  • Stem cells
  • Surface engineering
  • Wettability

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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