In vitro mesenchymal stem cell response to a CO2 laser modified polymeric material

D. G. Waugh; I. Hussain; J. Lawrence; G. C. Smith; D. Cosgrove; C. Toccaceli

doi:10.1016/j.msec.2016.05.055

In vitro mesenchymal stem cell response to a CO₂ laser modified polymeric material

D. G. Waugh, I. Hussain, J. Lawrence, G. C. Smith, D. Cosgrove, C. Toccaceli

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

94 Downloads (Pure)

Abstract

With an ageing world population it is becoming significantly apparent that there is a need to produce implants and platforms to manipulate stem cell growth on a pharmaceutical scale. This is needed to meet the socio-economic demands of many countries worldwide. This paper details one of the first ever studies in to the manipulation of stem cell growth on CO₂ laser surface treated nylon 6,6 highlighting its potential as an inexpensive platform to manipulate stem cell growth on a pharmaceutical scale. Through CO₂ laser surface treatment discrete changes to the surfaces were made. That is, the surface roughness of the nylon 6,6 was increased by up to 4.3 μm, the contact angle was modulated by up to 5Â° and the surface oxygen content increased by up to 1 atom %. Following mesenchymal stem cell growth on the laser treated samples, it was identified that CO₂ laser surface treatment gave rise to an enhanced response with an increase in viable cell count of up to 60,000 cells/ml when compared to the as-received sample. The effect of surface parameters modified by the CO₂ laser surface treatment on the mesenchymal stem cell response is also discussed along with potential trends that could be identified to govern the mesenchymal stem cell response.

Original language	English
Pages (from-to)	727-736
Number of pages	10
Journal	Materials Science and Engineering C
Volume	67
Early online date	16 May 2016
DOIs	https://doi.org/10.1016/j.msec.2016.05.055
Publication status	Published - 1 Oct 2016
Externally published	Yes

Bibliographical note

Publisher Statement: NOTICE: this is the author’s version of a work that was accepted for publication in Materials Science and Engineering C. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Science and Engineering C, [67 (2017)]DOI: 10.1016/j.msec.2016.05.055

http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

Adhesion
CO laser
Contact angle
Stem cells
Surface engineering
Wettability

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

Access to Document

10.1016/j.msec.2016.05.055

Post-PrintAccepted author manuscript, 1.53 MBLicence: CC BY-NC-ND

https://chesterrep.openrepository.com/handle/10034/611570

Cite this

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title = "In vitro mesenchymal stem cell response to a CO2 laser modified polymeric material",

abstract = "With an ageing world population it is becoming significantly apparent that there is a need to produce implants and platforms to manipulate stem cell growth on a pharmaceutical scale. This is needed to meet the socio-economic demands of many countries worldwide. This paper details one of the first ever studies in to the manipulation of stem cell growth on CO2 laser surface treated nylon 6,6 highlighting its potential as an inexpensive platform to manipulate stem cell growth on a pharmaceutical scale. Through CO2 laser surface treatment discrete changes to the surfaces were made. That is, the surface roughness of the nylon 6,6 was increased by up to 4.3 μm, the contact angle was modulated by up to 5{\^A}° and the surface oxygen content increased by up to 1 atom %. Following mesenchymal stem cell growth on the laser treated samples, it was identified that CO2 laser surface treatment gave rise to an enhanced response with an increase in viable cell count of up to 60,000 cells/ml when compared to the as-received sample. The effect of surface parameters modified by the CO2 laser surface treatment on the mesenchymal stem cell response is also discussed along with potential trends that could be identified to govern the mesenchymal stem cell response.",

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author = "Waugh, {D. G.} and I. Hussain and J. Lawrence and Smith, {G. C.} and D. Cosgrove and C. Toccaceli",

note = "Publisher Statement: NOTICE: this is the author{\textquoteright}s version of a work that was accepted for publication in Materials Science and Engineering C. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Science and Engineering C, [67 (2017)]DOI: 10.1016/j.msec.2016.05.055 http://creativecommons.org/licenses/by-nc-nd/4.0/ ",

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AU - Smith, G. C.

AU - Cosgrove, D.

AU - Toccaceli, C.

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