Impact of ageing and a synbiotic on the immune response to seasonal influenza vaccination; a randomised controlled trial

Sumia Enani, Agnieszka Przemska-Kosicka, Caroline E Childs, Catherine Maidens, Honglin Dong, Lorenza Conterno, Kieran Tuohy, Susan Todd, Margot Gosney, Parveen Yaqoob

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Abstract

Background & aims: Ageing increases risk of respiratory infections and impairs the response to influenza vaccination. Pre- and pro-biotics offer an opportunity to modulate anti-viral defenses and the response to vaccination via alteration of the gut microbiota. This study investigated the effect of a novel probiotic, Bifidobacterium longum bv. infantis CCUG 52486, combined with a prebiotic, gluco-oligosaccharide, on the B and T cell response to seasonal influenza vaccination in young and older subjects . Methods: In a double-blind, randomized controlled trial, 58 young (18e35 y) and 54 older (60e85 y) subjects were supplemented with the synbiotic for 8 weeks. At 4 weeks they were administered with a seasonal influenza vaccine. B and T cell phenotype and responsiveness to in vitro re-stimulation with the vaccine were assessed at baseline, 4, 6 and 8 weeks. Results: B and T cell profiles differed markedly between young and older subjects. Vaccination increased numbers of memory, IgAþ memory, IgGþ memory and total IgGþ B cells in young subjects, but failed to do so in older subjects and did not significantly alter T cell subsets. Seroconversion to the H1N1 subunit in the older subjects was associated with higher post-vaccination numbers of plasma B cells, but seroconversion was less consistently associated with T cell phenotype. B and T cell subsets from both young and older subjects demonstrated a strong antigen-specific recall challenge, and although not influenced by age, responsiveness to the recall challenge was associated with seroconversion. In older subjects, CMV seropositivity was associated with a significantly lower recall response to the vaccine, but the synbiotic did not affect the responsiveness of B or T cells to re-stimulation with influenza vaccine. Conclusions: Antigen-specific B and T cell activation following an in vitro recall challenge with the influenza vaccine was influenced by CMV seropositivity, but not by a synbiotic.
Original languageEnglish
Pages (from-to)443-451
Number of pages9
JournalClinical Nutrition
Volume37
Issue number2
Early online date28 Jan 2017
DOIs
Publication statusPublished - Apr 2018

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Synbiotics
Human Influenza
Vaccination
B-Lymphocytes
Randomized Controlled Trials
T-Lymphocytes
Influenza Vaccines
T-Lymphocyte Subsets
Vaccines
Immunoglobulin G
B-Lymphocyte Subsets
Phenotype
Antigens
Prebiotics
Probiotics
Plasma Cells
Oligosaccharides
Respiratory Tract Infections
Immunoglobulin A

Keywords

  • Ageing
  • Influenza
  • Probiotic
  • Lymphocyte
  • Vaccination

Cite this

Enani, S., Przemska-Kosicka, A., Childs, C. E., Maidens, C., Dong, H., Conterno, L., ... Yaqoob, P. (2018). Impact of ageing and a synbiotic on the immune response to seasonal influenza vaccination; a randomised controlled trial. Clinical Nutrition, 37(2), 443-451. https://doi.org/10.1016/j.clnu.2017.01.011

Impact of ageing and a synbiotic on the immune response to seasonal influenza vaccination; a randomised controlled trial. / Enani, Sumia ; Przemska-Kosicka, Agnieszka; Childs, Caroline E; Maidens, Catherine ; Dong, Honglin; Conterno, Lorenza; Tuohy, Kieran ; Todd, Susan; Gosney, Margot; Yaqoob, Parveen.

In: Clinical Nutrition, Vol. 37, No. 2, 04.2018, p. 443-451.

Research output: Contribution to journalArticle

Enani, S, Przemska-Kosicka, A, Childs, CE, Maidens, C, Dong, H, Conterno, L, Tuohy, K, Todd, S, Gosney, M & Yaqoob, P 2018, 'Impact of ageing and a synbiotic on the immune response to seasonal influenza vaccination; a randomised controlled trial' Clinical Nutrition, vol. 37, no. 2, pp. 443-451. https://doi.org/10.1016/j.clnu.2017.01.011
Enani, Sumia ; Przemska-Kosicka, Agnieszka ; Childs, Caroline E ; Maidens, Catherine ; Dong, Honglin ; Conterno, Lorenza ; Tuohy, Kieran ; Todd, Susan ; Gosney, Margot ; Yaqoob, Parveen. / Impact of ageing and a synbiotic on the immune response to seasonal influenza vaccination; a randomised controlled trial. In: Clinical Nutrition. 2018 ; Vol. 37, No. 2. pp. 443-451.
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AU - Childs, Caroline E

AU - Maidens, Catherine

AU - Dong, Honglin

AU - Conterno, Lorenza

AU - Tuohy, Kieran

AU - Todd, Susan

AU - Gosney, Margot

AU - Yaqoob, Parveen

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