Immunological control of the poultry red mite: The use of whole mite antigens as a vaccine candidate

Sam Arkle, David Harrington, Pete Kaiser, Lisa Rothwell, Carlos De Luna, David George, Jonathan Guy, Olivier A.E. Sparagano

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Citations (Scopus)

Abstract

In the current study whole poultry red mite antigens were extracted and birds were immunized subcutaneously with either antigen in adjuvant (antigen group) or PBS in adjuvant (control group). Immune responses of birds following immunization were investigated by ELISA and Western blotting, while vaccine efficacy was assessed by feeding of red mites on birds. Immunized birds showed a significant (P < 0.05) increase in IgY titers after immunization compared to controls, while immunoglobulin A (IgA) and IgM did not change significantly. However, the antigen group had a generally higher increase in all immunoglobulin titers compared to the controls. Western blotting identified a number of protein bands at different molecular weights, although these were not different between treatments. PCR analysis of whole mite protein identified bacterial DNA that might have confounded immunological data. In addition, there was a trend toward reduced survival rate of red mites feeding on antigen-immunized birds, but the difference was not statistically significant compared to controls. This study demonstrates the potential for somatic red mite antigens to stimulate an antibody-mediated immune response, although this response did not confer protection to birds.

Original languageEnglish
Title of host publicationAnimal Biodiversity and Emerging Diseases Prediction and Prevention
PublisherWiley
Pages36-40
Number of pages5
Volume1149
ISBN (Print)9781573317146
DOIs
Publication statusPublished - 15 Dec 2008
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1149
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Antigen
  • Chicken
  • Dermanyssus gallinae
  • Ectoparasite
  • Poultry red mite
  • Vaccine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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    Arkle, S., Harrington, D., Kaiser, P., Rothwell, L., De Luna, C., George, D., ... Sparagano, O. A. E. (2008). Immunological control of the poultry red mite: The use of whole mite antigens as a vaccine candidate. In Animal Biodiversity and Emerging Diseases Prediction and Prevention (Vol. 1149, pp. 36-40). (Annals of the New York Academy of Sciences; Vol. 1149). Wiley. https://doi.org/10.1196/annals.1428.057