Immunolocalization of keratan sulphate in the human embryonic cornea and other human foetal organs

L. Hyldahl, R. Aspinall, F. M. Watt

Research output: Contribution to journalArticle

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Abstract

An antibody raised against keratan sulphate was used to localize keratan sulphate in the human embryonic cornea and other human foetal organs. It was found that keratan sulphate was present in the corneal endothelium, as well as in the corneal stroma. In contrast, no keratan sulphate could be demonstrated in the corneal epithelium or in any posterior parts of the human foetal eye. Human corneal stromal cells grown in monolayers in tissue culture lost their capacity to bind this antibody. This suggests that explantation in vitro decreases or alters the expression of keratan sulphate in this cell type. Keratan sulphate was found to be present in abundant quantities in human embryonic cartilage and to a lesser extent in placenta. In contrast, keratan sulphate could not be detected in the foetal intestine, liver, suprarenal glands, the umbilical cord or in the foetal yolk sack.
Original languageEnglish
Pages (from-to)181-191
Number of pages11
JournalJournal of Cell Science
Volume80
Publication statusPublished - 1986
Externally publishedYes

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Keratan Sulfate
Cornea
Corneal Stroma
Corneal Endothelium
Corneal Epithelium
Antibodies
Umbilical Cord
Stromal Cells
Placenta
Cartilage
Intestines
Liver

ASJC Scopus subject areas

  • Cell Biology

Cite this

Immunolocalization of keratan sulphate in the human embryonic cornea and other human foetal organs. / Hyldahl, L.; Aspinall, R.; Watt, F. M.

In: Journal of Cell Science, Vol. 80, 1986, p. 181-191.

Research output: Contribution to journalArticle

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