Immunisation with recombinant proteins subolesin and Bm86 for the control of Dermanyssus gallinae in poultry

David Harrington; Mario Canales; José de la Fuente; Carlos de Luna; Karen Robinson; Jonathan Guy; Olivier Sparagano

doi:10.1016/j.vaccine.2009.04.014

Immunisation with recombinant proteins subolesin and Bm86 for the control of Dermanyssus gallinae in poultry

David Harrington
, Mario Canales
, José de la Fuente
, Carlos de Luna
, Karen Robinson
, Jonathan Guy
, Olivier Sparagano

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

Dermanyssus gallinae has a worldwide distribution and is considered to be the most serious and economically significant ectoparasite affecting egg-laying poultry in Europe. Recombinant Bm86 and subolesin proteins derived from Boophilus microplus ticks and Aedes albopictus mosquitoes were used to immunise poultry in an attempt to control D. gallinae in vitro. Immunisation with subolesin and Bm86 stimulated different profiles of IgY response, whilst Bm86 but not subolesin was recognized by IgY on western blots. Orthologues for Bm86 were not found in D. gallinae by PCR, but a 150 bp fragment aligned with mammalian akirin 1 and a 300 bp fragment aligned with Amblyomma hebraeum were amplified by subolesin PCR. D. gallinae mortality after feeding was 35.1% higher (P = 0.009) in the Subolesin group and 23% higher (not significant) in the Bm86 compared to the Control group. Thus it can be concluded that immunisation with recombinant subolesin can stimulate a protective response in laying hens against D. gallinae.

Original language	English
Pages (from-to)	4056-4063
Number of pages	8
Journal	Vaccine
Volume	27
Issue number	30
DOIs	https://doi.org/10.1016/j.vaccine.2009.04.014
Publication status	Published - 19 Jun 2009
Externally published	Yes

Funding

This work was supported by a grant from UK funding bodies the Biological and Biotechnology Research Council (BBSRC) and the Yorkshire Agricultural Society and from the INIA, Spain (project FAU2008-00014-00-00). We wish to thank all those at Newcastle University who helped in the conduct of the trial, especially Mr Hatem Mohi El Din, Mr Jim Wightman and finally Dr Robert Shiel for statistical advice.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Bm86
Dermanyssus gallinae
Ectoparasite
Poultry
Subolesin
Vaccination

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

Access to Document

10.1016/j.vaccine.2009.04.014

Cite this

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title = "Immunisation with recombinant proteins subolesin and Bm86 for the control of Dermanyssus gallinae in poultry",

abstract = "Dermanyssus gallinae has a worldwide distribution and is considered to be the most serious and economically significant ectoparasite affecting egg-laying poultry in Europe. Recombinant Bm86 and subolesin proteins derived from Boophilus microplus ticks and Aedes albopictus mosquitoes were used to immunise poultry in an attempt to control D. gallinae in vitro. Immunisation with subolesin and Bm86 stimulated different profiles of IgY response, whilst Bm86 but not subolesin was recognized by IgY on western blots. Orthologues for Bm86 were not found in D. gallinae by PCR, but a 150 bp fragment aligned with mammalian akirin 1 and a 300 bp fragment aligned with Amblyomma hebraeum were amplified by subolesin PCR. D. gallinae mortality after feeding was 35.1\% higher (P = 0.009) in the Subolesin group and 23\% higher (not significant) in the Bm86 compared to the Control group. Thus it can be concluded that immunisation with recombinant subolesin can stimulate a protective response in laying hens against D. gallinae.",

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AU - Harrington, David

AU - Canales, Mario

AU - de la Fuente, José

AU - de Luna, Carlos

AU - Robinson, Karen

AU - Guy, Jonathan

AU - Sparagano, Olivier

PY - 2009/6/19

Y1 - 2009/6/19

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AB - Dermanyssus gallinae has a worldwide distribution and is considered to be the most serious and economically significant ectoparasite affecting egg-laying poultry in Europe. Recombinant Bm86 and subolesin proteins derived from Boophilus microplus ticks and Aedes albopictus mosquitoes were used to immunise poultry in an attempt to control D. gallinae in vitro. Immunisation with subolesin and Bm86 stimulated different profiles of IgY response, whilst Bm86 but not subolesin was recognized by IgY on western blots. Orthologues for Bm86 were not found in D. gallinae by PCR, but a 150 bp fragment aligned with mammalian akirin 1 and a 300 bp fragment aligned with Amblyomma hebraeum were amplified by subolesin PCR. D. gallinae mortality after feeding was 35.1% higher (P = 0.009) in the Subolesin group and 23% higher (not significant) in the Bm86 compared to the Control group. Thus it can be concluded that immunisation with recombinant subolesin can stimulate a protective response in laying hens against D. gallinae.

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KW - Dermanyssus gallinae

KW - Ectoparasite

KW - Poultry

KW - Subolesin

KW - Vaccination

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ER -

Immunisation with recombinant proteins subolesin and Bm86 for the control of Dermanyssus gallinae in poultry

Abstract

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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