Identification of Small-Molecule Positive Modulators of Calcitonin-like Receptor-Based Receptors

Erica R. Hendrikse, Lydia P. Liew, Rebekah L. Bower, Muriel Bonnet, Muhammad A. Jamaluddin, Nicole Prodan, Keith D. Richards, Christopher S. Walker, Garry Pairaudeau, David M. Smith, Roxana Maria Rujan, Risha Sudra, Christopher A. Reynolds, Jason M. Booe, Augen A. Pioszak, Jack U. Flanagan, Michael P. Hay, Debbie L. Hay

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Class B G protein-coupled receptors are highly therapeutically relevant but challenges remain in identifying suitable small-molecule drugs. The calcitonin-like receptor (CLR) in particular is linked to conditions such as migraine, cardiovascular disease, and inflammatory bowel disease. The CLR cannot act as a cell-surface receptor alone but rather must couple to one of three receptor activity-modifying proteins (RAMPs), forming heterodimeric receptors for the peptides adrenomedullin and calcitonin gene-related peptide. These peptides have extended binding sites across their receptors. This is one reason why there are few small-molecule ligands that can modulate these receptors. Here we describe small molecules that are able to positively modulate the signaling of the CLR with all three RAMPs but are not active at the related calcitonin receptor. These compounds were selected from a β-arrestin recruitment screen, coupled with rounds of medicinal chemistry to improve their activity. Translational potential is shown as the compounds can positively modulate cAMP signaling in a vascular cell line model. Binding experiments do not support an extracellular domain binding site; however, molecular modeling reveals potential allosteric binding sites in multiple receptor regions. These are the first small-molecule positive modulators described for the CLR:RAMP complexes.

Original languageEnglish
Pages (from-to)305-320
Number of pages16
JournalACS Pharmacology and Translational Science
Volume3
Issue number2
Early online date12 Mar 2020
DOIs
Publication statusPublished - 10 Apr 2020
Externally publishedYes

Keywords

  • adrenomedullin
  • allosteric modulator
  • calcitonin gene-related peptide
  • G protein-coupled receptor
  • receptor activity-modifying protein

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology

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  • Cite this

    Hendrikse, E. R., Liew, L. P., Bower, R. L., Bonnet, M., Jamaluddin, M. A., Prodan, N., ... Hay, D. L. (2020). Identification of Small-Molecule Positive Modulators of Calcitonin-like Receptor-Based Receptors. ACS Pharmacology and Translational Science, 3(2), 305-320. https://doi.org/10.1021/acsptsci.9b00108