Identification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans

H J Sears, B Bennett, S Spiro, A J Thomson, D J Richardson

Research output: Contribution to journalArticle

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Abstract

EPR spectroscopy has been successfully used to detect signals due to molybdenum (V) and ferric iron in intact cells of aerobically grown Paracoccus denitrificans. The signals are ascribed to the catalytic molybdenum centre and to the haem iron of the periplasmic nitrate reductase. These signals are absent from a mutant strain deficient in this enzyme. The Mo(V) signal is due to the High-g Split species which has been well characterized in the purified enzyme. This confirms that the High-g Split is the physiologically relevant signal of a number observed in the previous work on the purified enzyme.

Original languageEnglish
Pages (from-to)311-4
Number of pages4
JournalBiochemical Journal
Volume310 ( Pt 1)
Publication statusPublished - 15 Aug 1995
Externally publishedYes

Fingerprint

Paracoccus denitrificans
Nitrate Reductase
Paramagnetic resonance
Molybdenum
Enzymes
Iron
Heme
Spectrum Analysis
Spectroscopy

Keywords

  • Cold Temperature
  • Electron Spin Resonance Spectroscopy
  • Nitrate Reductase
  • Nitrate Reductases
  • Paracoccus denitrificans
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Sears, H. J., Bennett, B., Spiro, S., Thomson, A. J., & Richardson, D. J. (1995). Identification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans. Biochemical Journal, 310 ( Pt 1), 311-4.

Identification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans. / Sears, H J; Bennett, B; Spiro, S; Thomson, A J; Richardson, D J.

In: Biochemical Journal, Vol. 310 ( Pt 1), 15.08.1995, p. 311-4.

Research output: Contribution to journalArticle

Sears, HJ, Bennett, B, Spiro, S, Thomson, AJ & Richardson, DJ 1995, 'Identification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans' Biochemical Journal, vol. 310 ( Pt 1), pp. 311-4.
Sears, H J ; Bennett, B ; Spiro, S ; Thomson, A J ; Richardson, D J. / Identification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans. In: Biochemical Journal. 1995 ; Vol. 310 ( Pt 1). pp. 311-4.
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abstract = "EPR spectroscopy has been successfully used to detect signals due to molybdenum (V) and ferric iron in intact cells of aerobically grown Paracoccus denitrificans. The signals are ascribed to the catalytic molybdenum centre and to the haem iron of the periplasmic nitrate reductase. These signals are absent from a mutant strain deficient in this enzyme. The Mo(V) signal is due to the High-g Split species which has been well characterized in the purified enzyme. This confirms that the High-g Split is the physiologically relevant signal of a number observed in the previous work on the purified enzyme.",
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AU - Thomson, A J

AU - Richardson, D J

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N2 - EPR spectroscopy has been successfully used to detect signals due to molybdenum (V) and ferric iron in intact cells of aerobically grown Paracoccus denitrificans. The signals are ascribed to the catalytic molybdenum centre and to the haem iron of the periplasmic nitrate reductase. These signals are absent from a mutant strain deficient in this enzyme. The Mo(V) signal is due to the High-g Split species which has been well characterized in the purified enzyme. This confirms that the High-g Split is the physiologically relevant signal of a number observed in the previous work on the purified enzyme.

AB - EPR spectroscopy has been successfully used to detect signals due to molybdenum (V) and ferric iron in intact cells of aerobically grown Paracoccus denitrificans. The signals are ascribed to the catalytic molybdenum centre and to the haem iron of the periplasmic nitrate reductase. These signals are absent from a mutant strain deficient in this enzyme. The Mo(V) signal is due to the High-g Split species which has been well characterized in the purified enzyme. This confirms that the High-g Split is the physiologically relevant signal of a number observed in the previous work on the purified enzyme.

KW - Cold Temperature

KW - Electron Spin Resonance Spectroscopy

KW - Nitrate Reductase

KW - Nitrate Reductases

KW - Paracoccus denitrificans

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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SN - 0264-6021

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