Hyperpolarized butyrate: A metabolic probe of short chain fatty acid metabolism in the heart

Daniel R. Ball, Ben Rowlands, Michael S. Dodd, Lydia Le Page, Vicky Ball, Carolyn A. Carr, Kieran Clarke, Damian J. Tyler

Research output: Contribution to journalArticle

42 Citations (Scopus)
10 Downloads (Pure)

Abstract

Purpose Butyrate, a short chain fatty acid, was studied as a novel hyperpolarized substrate for use in dynamic nuclear polarization enhanced magnetic resonance spectroscopy experiments, to define the pathways of short chain fatty acid and ketone body metabolism in real time. Methods Butyrate was polarized via the dynamic nuclear polarization process and subsequently dissolved to generate an injectable metabolic substrate. Metabolism was initially assessed in the isolated perfused rat heart, followed by evaluation in the in vivo rat heart. Results Hyperpolarized butyrate was generated with a polarization level of 7% and was shown to have a T1 relaxation time of 20 s. These physical characteristics were sufficient to enable assessment of multiple steps in its metabolism, with the ketone body acetoacetate and several tricarboxylic acid cycle intermediates observed both in vitro and in vivo. Metabolite to butyrate ratios of 0.1-0.4% and 0.5-2% were observed in vitro and in vivo respectively, similar to levels previously observed with hyperpolarized [2-13C]pyruvate. Conclusions In this study, butyrate has been demonstrated to be a suitable hyperpolarized substrate capable of revealing multi-step metabolism in dynamic nuclear polarization experiments and providing information on the metabolism of fatty acids not currently achievable with other hyperpolarized substrates.

Original languageEnglish
Pages (from-to)1663-1669
Number of pages7
JournalMagnetic Resonance in Medicine
Volume71
Issue number5
Publication statusPublished - 24 Jun 2013
Externally publishedYes

Fingerprint

Volatile Fatty Acids
Butyrates
Ketone Bodies
Citric Acid Cycle
Pyruvic Acid
Magnetic Resonance Spectroscopy
Fatty Acids
Injections
In Vitro Techniques

Keywords

  • butyrate
  • dynamic nuclear polarization
  • heart
  • hyperpolarization
  • ketone bodies
  • magnetic resonance spectroscopy
  • short chain fatty acids

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

Cite this

Ball, D. R., Rowlands, B., Dodd, M. S., Le Page, L., Ball, V., Carr, C. A., ... Tyler, D. J. (2013). Hyperpolarized butyrate: A metabolic probe of short chain fatty acid metabolism in the heart. Magnetic Resonance in Medicine, 71(5), 1663-1669.

Hyperpolarized butyrate : A metabolic probe of short chain fatty acid metabolism in the heart. / Ball, Daniel R.; Rowlands, Ben; Dodd, Michael S.; Le Page, Lydia; Ball, Vicky; Carr, Carolyn A.; Clarke, Kieran; Tyler, Damian J.

In: Magnetic Resonance in Medicine, Vol. 71, No. 5, 24.06.2013, p. 1663-1669.

Research output: Contribution to journalArticle

Ball, DR, Rowlands, B, Dodd, MS, Le Page, L, Ball, V, Carr, CA, Clarke, K & Tyler, DJ 2013, 'Hyperpolarized butyrate: A metabolic probe of short chain fatty acid metabolism in the heart' Magnetic Resonance in Medicine, vol. 71, no. 5, pp. 1663-1669.
Ball, Daniel R. ; Rowlands, Ben ; Dodd, Michael S. ; Le Page, Lydia ; Ball, Vicky ; Carr, Carolyn A. ; Clarke, Kieran ; Tyler, Damian J. / Hyperpolarized butyrate : A metabolic probe of short chain fatty acid metabolism in the heart. In: Magnetic Resonance in Medicine. 2013 ; Vol. 71, No. 5. pp. 1663-1669.
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