Hydrophobically-modified chitosan nanoliposomes for intestinal drug delivery

Mohammed Gulrez Zariwala; Harshada Bendre; Anatoliy Markiv; Sebastien Farnaud; Derek Renshaw; Kevin M G Taylor; Satyanarayana Somavarapu

doi:10.2147/IJN.S166901

Hydrophobically-modified chitosan nanoliposomes for intestinal drug delivery

Mohammed Gulrez Zariwala
, Harshada Bendre
, Anatoliy Markiv
, Sebastien Farnaud
, Derek Renshaw
, Kevin M G Taylor
, Satyanarayana Somavarapu

University of Westminster
King's College London
University College London

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

166 Downloads (Pure)

Abstract

A novel chitosan derivative, O-palmitoyl chitosan (OPC) was synthesized from chitosan and palmitoyl chloride using methane-sulfonic acid as a solvent. The success of synthesis was confirmed by Fourier transform infra-red (FT-IR) spectroscopy and proton NMR spectroscopy (H-NMR). Liposomes encapsulating ferrous sulphate as a model hydrophilic drug for intestinal delivery were prepared with or without OPC inclusion (Lipo-Fe and OPC-Lipo-Fe). Entrapment of iron was significantly higher in OPC containing liposomes compared to controls. Quantitative iron absorption from the OPC liposomes was significantly higher (1.5-fold P< 0.05) than free ferrous sulphate controls. Qualitative uptake analysis by confocal imaging using coumarin-6 dye loaded liposomes also indicated higher cellular uptake and internalization of the OPC-containing liposomes. These findings suggest that addition of OPC during liposome preparation creates robust vesicles that have improved mucoadhesive and absorption enhancing properties. The chitosan derivative OPC therefore provides a novel alternative for formulation of delivery vehicles targeting intestinal absorption.

Original language	English
Pages (from-to)	5837-5848
Number of pages	12
Journal	International Journal of Nanomedicine
Volume	13
DOIs	https://doi.org/10.2147/IJN.S166901
Publication status	Published - 27 Sept 2018

Bibliographical note

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

Keywords

Liposomes
Ferrous sulphate
Caco-2
Gut delivery
Intestinal absorption

ASJC Scopus subject areas

Pharmacology, Toxicology and Pharmaceutics(all)

Access to Document

10.2147/IJN.S166901Licence: CC BY-NC

PublishedFinal published version, 2.63 MBLicence: CC BY-NC

New capsule could provide remedy for iron deficiency
Farnaud, S.
16/04/14
1 Media contribution
Press/Media: Other

Cite this

@article{2adfdc2d71ae4013aa75c3446c4a4f0a,

title = "Hydrophobically-modified chitosan nanoliposomes for intestinal drug delivery",

abstract = "A novel chitosan derivative, O-palmitoyl chitosan (OPC) was synthesized from chitosan and palmitoyl chloride using methane-sulfonic acid as a solvent. The success of synthesis was confirmed by Fourier transform infra-red (FT-IR) spectroscopy and proton NMR spectroscopy (H-NMR). Liposomes encapsulating ferrous sulphate as a model hydrophilic drug for intestinal delivery were prepared with or without OPC inclusion (Lipo-Fe and OPC-Lipo-Fe). Entrapment of iron was significantly higher in OPC containing liposomes compared to controls. Quantitative iron absorption from the OPC liposomes was significantly higher (1.5-fold P< 0.05) than free ferrous sulphate controls. Qualitative uptake analysis by confocal imaging using coumarin-6 dye loaded liposomes also indicated higher cellular uptake and internalization of the OPC-containing liposomes. These findings suggest that addition of OPC during liposome preparation creates robust vesicles that have improved mucoadhesive and absorption enhancing properties. The chitosan derivative OPC therefore provides a novel alternative for formulation of delivery vehicles targeting intestinal absorption.",

keywords = "Liposomes, Ferrous sulphate, Caco-2, Gut delivery, Intestinal absorption",

author = "Zariwala, \{Mohammed Gulrez\} and Harshada Bendre and Anatoliy Markiv and Sebastien Farnaud and Derek Renshaw and Taylor, \{Kevin M G\} and Satyanarayana Somavarapu",

note = "This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.",

year = "2018",

month = sep,

day = "27",

doi = "10.2147/IJN.S166901",

language = "English",

volume = "13",

pages = "5837--5848",

journal = "International Journal of Nanomedicine",

issn = "1178-2013",

publisher = "Dove Medical Press Ltd.",

}

TY - JOUR

T1 - Hydrophobically-modified chitosan nanoliposomes for intestinal drug delivery

AU - Zariwala, Mohammed Gulrez

AU - Bendre, Harshada

AU - Markiv, Anatoliy

AU - Farnaud, Sebastien

AU - Renshaw, Derek

AU - Taylor, Kevin M G

AU - Somavarapu, Satyanarayana

N1 - This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PY - 2018/9/27

Y1 - 2018/9/27

N2 - A novel chitosan derivative, O-palmitoyl chitosan (OPC) was synthesized from chitosan and palmitoyl chloride using methane-sulfonic acid as a solvent. The success of synthesis was confirmed by Fourier transform infra-red (FT-IR) spectroscopy and proton NMR spectroscopy (H-NMR). Liposomes encapsulating ferrous sulphate as a model hydrophilic drug for intestinal delivery were prepared with or without OPC inclusion (Lipo-Fe and OPC-Lipo-Fe). Entrapment of iron was significantly higher in OPC containing liposomes compared to controls. Quantitative iron absorption from the OPC liposomes was significantly higher (1.5-fold P< 0.05) than free ferrous sulphate controls. Qualitative uptake analysis by confocal imaging using coumarin-6 dye loaded liposomes also indicated higher cellular uptake and internalization of the OPC-containing liposomes. These findings suggest that addition of OPC during liposome preparation creates robust vesicles that have improved mucoadhesive and absorption enhancing properties. The chitosan derivative OPC therefore provides a novel alternative for formulation of delivery vehicles targeting intestinal absorption.

AB - A novel chitosan derivative, O-palmitoyl chitosan (OPC) was synthesized from chitosan and palmitoyl chloride using methane-sulfonic acid as a solvent. The success of synthesis was confirmed by Fourier transform infra-red (FT-IR) spectroscopy and proton NMR spectroscopy (H-NMR). Liposomes encapsulating ferrous sulphate as a model hydrophilic drug for intestinal delivery were prepared with or without OPC inclusion (Lipo-Fe and OPC-Lipo-Fe). Entrapment of iron was significantly higher in OPC containing liposomes compared to controls. Quantitative iron absorption from the OPC liposomes was significantly higher (1.5-fold P< 0.05) than free ferrous sulphate controls. Qualitative uptake analysis by confocal imaging using coumarin-6 dye loaded liposomes also indicated higher cellular uptake and internalization of the OPC-containing liposomes. These findings suggest that addition of OPC during liposome preparation creates robust vesicles that have improved mucoadhesive and absorption enhancing properties. The chitosan derivative OPC therefore provides a novel alternative for formulation of delivery vehicles targeting intestinal absorption.

KW - Liposomes

KW - Ferrous sulphate

KW - Caco-2

KW - Gut delivery

KW - Intestinal absorption

U2 - 10.2147/IJN.S166901

DO - 10.2147/IJN.S166901

M3 - Article

SN - 1178-2013

VL - 13

SP - 5837

EP - 5848

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

ER -

Hydrophobically-modified chitosan nanoliposomes for intestinal drug delivery

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Press/Media

New capsule could provide remedy for iron deficiency

Cite this