A novel chitosan derivative, O-palmitoyl chitosan (OPC) was synthesized from chitosan and palmitoyl chloride using methane-sulfonic acid as a solvent. The success of synthesis was confirmed by Fourier transform infra-red (FT-IR) spectroscopy and proton NMR spectroscopy (H-NMR). Liposomes encapsulating ferrous sulphate as a model hydrophilic drug for intestinal delivery were prepared with or without OPC inclusion (Lipo-Fe and OPC-Lipo-Fe). Entrapment of iron was significantly higher in OPC containing liposomes compared to controls. Quantitative iron absorption from the OPC liposomes was significantly higher (1.5-fold P< 0.05) than free ferrous sulphate controls. Qualitative uptake analysis by confocal imaging using coumarin-6 dye loaded liposomes also indicated higher cellular uptake and internalization of the OPC-containing liposomes. These findings suggest that addition of OPC during liposome preparation creates robust vesicles that have improved mucoadhesive and absorption enhancing properties. The chitosan derivative OPC therefore provides a novel alternative for formulation of delivery vehicles targeting intestinal absorption.
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- Ferrous sulphate
- Gut delivery
- Intestinal absorption
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics(all)
Zariwala, M. G., Bendre, H., Markiv, A., Farnaud, S., Renshaw, D., Taylor, K. M. G., & Somavarapu, S. (2018). Hydrophobically-modified chitosan nanoliposomes for intestinal drug delivery. International Journal of Nanomedicine, 13, 5837-5848. https://doi.org/10.2147/IJN.S166901