Hepatocyte growth factor (HGF/SF) is expressed in human epithelial cells during embryonic development; Studies by in situ hybridisation and Northern blot analysis

Y. Wang, C. Selden, S. Farnaud, D. Calnan, H. J.F. Hodgson

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

This study reports the tissue distribution of Hepatocyte Growth Factor-Scatter Factor (HGF/SF) in human fetal tissue using Northern analysis and in situ hybridisation techniques. In tissue from fetuses of 9-17 wk gestational age, the 6 kb mRNA transcript for HGF/SF was demonstrated in many tissues but prominently in liver, intestine, gall bladder and spleen. In situ hybridisation demonstrated that HGF/SF expression was not confined to mesenchymal tissues, as suggested by previous studies but was expressed in epithelial tissues, particularly in small intestine, keratinising epithelium of tongue, skin and oesophagus. In the small intestine epithelial expression was strikingly regional, being confined to the crypt region, the site of enterocyte proliferation. Northern analysis of tissues for c-met mRNA, representing expression of the HGF/SF receptor, demonstrated receptor expression in all tissues studied except the thyroid gland.

Original languageEnglish
Pages (from-to)543-551
Number of pages9
JournalJournal of Anatomy
Volume185
Issue number3
Publication statusPublished - 1994
Externally publishedYes

Keywords

  • Fetal development
  • Hepatocyte growth factor receptor
  • Scatter factor

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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