Genetic modification of primary chronic lymphocytic leukemia cells with a lentivirus expressing CD38.

L. Pearce, L. Morgan, Thet Thet Lin, Saman Hewamana, R. James Matthews, Silvia Deaglio, Clare Rowntree, Chris Fegan, Chris Pepper, Paul Brennan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Studies of the role of individual genes in chronic lymphocytic leukemia (CLL) have been hampered by the inability to consistently transfect primary tumor cells. Here, we describe a highly efficient method of genetically modifying primary CLL cells using a VSVG pseudotyped lentiviral vector. We transduced CD38 negative CLL cells with a lentiviral vector encoding CD38 which caused increased surface CD38 expression in all the samples tested (n=17) with no evidence of plasmacytoid differentiation. The mean percentage of positive cells expressing CD38 was 87%±8.5% and the mean cell viability 74%±17%. This high level of transduction of all the CLL cell samples tested demonstrates the utility of this technique which should prove applicable for the introduction and analysis of other genes in these non-dividing cells.
Original languageEnglish
Pages (from-to)514-517
Number of pages4
JournalHaematologica
Volume95
Issue number3
DOIs
Publication statusPublished - Mar 2010
Externally publishedYes

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