Fruits, vegetables, and hMLH1 protein-deficient and -proficient colon cancer: The Netherlands cohort study

Petra A. Wark, Matty P. Weijenberg, Pieter Van't Veer, Gerda Van Wijhe, Margreet Luchtenborg, Goos N P Van Muijen, Anton F P M De Goeij, R. Alexandra Goldbohm, Piet A. Van Den Brandt

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Background: Clinical and pathologic differences exist between colon carcinomas deficient and proficient in the mismatch repair protein hMLH1. Animal and in vitro studies suggest that fruits, vegetables, folate, and antioxidants are associated with colonic expression of mismatch repair genes.

    Methods: Associations between consumption of fruits and vegetables and hMLH1 protein-deficient and -proficient colon cancer were evaluated in the Netherlands Cohort Study on diet and cancer using a case-cohort approach. A self-administered food frequency questionnaire was completed, in 1986, by 120,852 individuals ages 55 to 69 years. Using immunohistochemistry, hMLH1 protein expression was assessed in colon cancer tissue obtained from 441 patients who were identified over 7.3 years of follow-up excluding the initial 2.3 years. Incidence rate ratios (RR) were estimated for hMLH1 protein-deficient and -proficient colon cancer.

    Results: hMLH1 protein expression was absent in 54 tumors (12.2%) and present in 387 tumors. Fruit consumption was associated with hMLH1 protein-deficient colon cancer [highest versus lowest tertile, RR, 0.46; 95% confidence interval (95% CI), 0.23-0.90; Ptrend = 0.029] but not with hMLH1 protein-proficient tumors (highest versus lowest tertile, RR, 1.03; 95% CI, 0.78-1.35; Ptrend = 0.81). Total consumption of vegetables was not associated with either type of tumor (hMLH1 protein deficient: RR, 0.86; 95% CI, 0.45-1.65; Ptrend = 0.67; hMLH1 protein proficient: RR, 0.94; 95% CI, 0.72-1.23; Ptrend = 0.72). No associations were observed for folate, fiber, antioxidants, or subgroups of vegetables. 

    Conclusion: These analyses indicate that an inverse association between consumption of fruits and colon cancer may be confined to the subgroup of tumors with a deficient mismatch repair system.

    Original languageEnglish
    Pages (from-to)1619-1625
    Number of pages7
    JournalCancer Epidemiology Biomarkers and Prevention
    Volume14
    Issue number7
    DOIs
    Publication statusPublished - Jul 2005

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    Vegetable Proteins
    Netherlands
    Colonic Neoplasms
    Fruit
    Cohort Studies
    DNA Mismatch Repair
    Proteins
    Vegetables
    Confidence Intervals
    Neoplasms
    Folic Acid
    Antioxidants
    Colon
    Immunohistochemistry
    Diet
    Carcinoma
    Food

    ASJC Scopus subject areas

    • Epidemiology
    • Oncology

    Cite this

    Wark, P. A., Weijenberg, M. P., Van't Veer, P., Van Wijhe, G., Luchtenborg, M., Van Muijen, G. N. P., ... Van Den Brandt, P. A. (2005). Fruits, vegetables, and hMLH1 protein-deficient and -proficient colon cancer: The Netherlands cohort study. Cancer Epidemiology Biomarkers and Prevention, 14(7), 1619-1625. https://doi.org/10.1158/1055-9965.EPI-05-0109

    Fruits, vegetables, and hMLH1 protein-deficient and -proficient colon cancer : The Netherlands cohort study. / Wark, Petra A.; Weijenberg, Matty P.; Van't Veer, Pieter; Van Wijhe, Gerda; Luchtenborg, Margreet; Van Muijen, Goos N P; De Goeij, Anton F P M; Goldbohm, R. Alexandra; Van Den Brandt, Piet A.

    In: Cancer Epidemiology Biomarkers and Prevention, Vol. 14, No. 7, 07.2005, p. 1619-1625.

    Research output: Contribution to journalArticle

    Wark, PA, Weijenberg, MP, Van't Veer, P, Van Wijhe, G, Luchtenborg, M, Van Muijen, GNP, De Goeij, AFPM, Goldbohm, RA & Van Den Brandt, PA 2005, 'Fruits, vegetables, and hMLH1 protein-deficient and -proficient colon cancer: The Netherlands cohort study' Cancer Epidemiology Biomarkers and Prevention, vol. 14, no. 7, pp. 1619-1625. https://doi.org/10.1158/1055-9965.EPI-05-0109
    Wark, Petra A. ; Weijenberg, Matty P. ; Van't Veer, Pieter ; Van Wijhe, Gerda ; Luchtenborg, Margreet ; Van Muijen, Goos N P ; De Goeij, Anton F P M ; Goldbohm, R. Alexandra ; Van Den Brandt, Piet A. / Fruits, vegetables, and hMLH1 protein-deficient and -proficient colon cancer : The Netherlands cohort study. In: Cancer Epidemiology Biomarkers and Prevention. 2005 ; Vol. 14, No. 7. pp. 1619-1625.
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    title = "Fruits, vegetables, and hMLH1 protein-deficient and -proficient colon cancer: The Netherlands cohort study",
    abstract = "Background: Clinical and pathologic differences exist between colon carcinomas deficient and proficient in the mismatch repair protein hMLH1. Animal and in vitro studies suggest that fruits, vegetables, folate, and antioxidants are associated with colonic expression of mismatch repair genes.Methods: Associations between consumption of fruits and vegetables and hMLH1 protein-deficient and -proficient colon cancer were evaluated in the Netherlands Cohort Study on diet and cancer using a case-cohort approach. A self-administered food frequency questionnaire was completed, in 1986, by 120,852 individuals ages 55 to 69 years. Using immunohistochemistry, hMLH1 protein expression was assessed in colon cancer tissue obtained from 441 patients who were identified over 7.3 years of follow-up excluding the initial 2.3 years. Incidence rate ratios (RR) were estimated for hMLH1 protein-deficient and -proficient colon cancer.Results: hMLH1 protein expression was absent in 54 tumors (12.2{\%}) and present in 387 tumors. Fruit consumption was associated with hMLH1 protein-deficient colon cancer [highest versus lowest tertile, RR, 0.46; 95{\%} confidence interval (95{\%} CI), 0.23-0.90; Ptrend = 0.029] but not with hMLH1 protein-proficient tumors (highest versus lowest tertile, RR, 1.03; 95{\%} CI, 0.78-1.35; Ptrend = 0.81). Total consumption of vegetables was not associated with either type of tumor (hMLH1 protein deficient: RR, 0.86; 95{\%} CI, 0.45-1.65; Ptrend = 0.67; hMLH1 protein proficient: RR, 0.94; 95{\%} CI, 0.72-1.23; Ptrend = 0.72). No associations were observed for folate, fiber, antioxidants, or subgroups of vegetables. Conclusion: These analyses indicate that an inverse association between consumption of fruits and colon cancer may be confined to the subgroup of tumors with a deficient mismatch repair system.",
    author = "Wark, {Petra A.} and Weijenberg, {Matty P.} and {Van't Veer}, Pieter and {Van Wijhe}, Gerda and Margreet Luchtenborg and {Van Muijen}, {Goos N P} and {De Goeij}, {Anton F P M} and Goldbohm, {R. Alexandra} and {Van Den Brandt}, {Piet A.}",
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    TY - JOUR

    T1 - Fruits, vegetables, and hMLH1 protein-deficient and -proficient colon cancer

    T2 - The Netherlands cohort study

    AU - Wark, Petra A.

    AU - Weijenberg, Matty P.

    AU - Van't Veer, Pieter

    AU - Van Wijhe, Gerda

    AU - Luchtenborg, Margreet

    AU - Van Muijen, Goos N P

    AU - De Goeij, Anton F P M

    AU - Goldbohm, R. Alexandra

    AU - Van Den Brandt, Piet A.

    PY - 2005/7

    Y1 - 2005/7

    N2 - Background: Clinical and pathologic differences exist between colon carcinomas deficient and proficient in the mismatch repair protein hMLH1. Animal and in vitro studies suggest that fruits, vegetables, folate, and antioxidants are associated with colonic expression of mismatch repair genes.Methods: Associations between consumption of fruits and vegetables and hMLH1 protein-deficient and -proficient colon cancer were evaluated in the Netherlands Cohort Study on diet and cancer using a case-cohort approach. A self-administered food frequency questionnaire was completed, in 1986, by 120,852 individuals ages 55 to 69 years. Using immunohistochemistry, hMLH1 protein expression was assessed in colon cancer tissue obtained from 441 patients who were identified over 7.3 years of follow-up excluding the initial 2.3 years. Incidence rate ratios (RR) were estimated for hMLH1 protein-deficient and -proficient colon cancer.Results: hMLH1 protein expression was absent in 54 tumors (12.2%) and present in 387 tumors. Fruit consumption was associated with hMLH1 protein-deficient colon cancer [highest versus lowest tertile, RR, 0.46; 95% confidence interval (95% CI), 0.23-0.90; Ptrend = 0.029] but not with hMLH1 protein-proficient tumors (highest versus lowest tertile, RR, 1.03; 95% CI, 0.78-1.35; Ptrend = 0.81). Total consumption of vegetables was not associated with either type of tumor (hMLH1 protein deficient: RR, 0.86; 95% CI, 0.45-1.65; Ptrend = 0.67; hMLH1 protein proficient: RR, 0.94; 95% CI, 0.72-1.23; Ptrend = 0.72). No associations were observed for folate, fiber, antioxidants, or subgroups of vegetables. Conclusion: These analyses indicate that an inverse association between consumption of fruits and colon cancer may be confined to the subgroup of tumors with a deficient mismatch repair system.

    AB - Background: Clinical and pathologic differences exist between colon carcinomas deficient and proficient in the mismatch repair protein hMLH1. Animal and in vitro studies suggest that fruits, vegetables, folate, and antioxidants are associated with colonic expression of mismatch repair genes.Methods: Associations between consumption of fruits and vegetables and hMLH1 protein-deficient and -proficient colon cancer were evaluated in the Netherlands Cohort Study on diet and cancer using a case-cohort approach. A self-administered food frequency questionnaire was completed, in 1986, by 120,852 individuals ages 55 to 69 years. Using immunohistochemistry, hMLH1 protein expression was assessed in colon cancer tissue obtained from 441 patients who were identified over 7.3 years of follow-up excluding the initial 2.3 years. Incidence rate ratios (RR) were estimated for hMLH1 protein-deficient and -proficient colon cancer.Results: hMLH1 protein expression was absent in 54 tumors (12.2%) and present in 387 tumors. Fruit consumption was associated with hMLH1 protein-deficient colon cancer [highest versus lowest tertile, RR, 0.46; 95% confidence interval (95% CI), 0.23-0.90; Ptrend = 0.029] but not with hMLH1 protein-proficient tumors (highest versus lowest tertile, RR, 1.03; 95% CI, 0.78-1.35; Ptrend = 0.81). Total consumption of vegetables was not associated with either type of tumor (hMLH1 protein deficient: RR, 0.86; 95% CI, 0.45-1.65; Ptrend = 0.67; hMLH1 protein proficient: RR, 0.94; 95% CI, 0.72-1.23; Ptrend = 0.72). No associations were observed for folate, fiber, antioxidants, or subgroups of vegetables. Conclusion: These analyses indicate that an inverse association between consumption of fruits and colon cancer may be confined to the subgroup of tumors with a deficient mismatch repair system.

    U2 - 10.1158/1055-9965.EPI-05-0109

    DO - 10.1158/1055-9965.EPI-05-0109

    M3 - Article

    VL - 14

    SP - 1619

    EP - 1625

    JO - Cancer Epidemiology Biomarkers and Prevention

    JF - Cancer Epidemiology Biomarkers and Prevention

    SN - 1055-9965

    IS - 7

    ER -