Fibril formation from pea protein and subsequent gel formation

Claire Darizu Munialo, Anneke H. Martin, Erik Van Der Linden, Harmen H.J. De Jongh

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The objective of this study was to characterize fibrillar aggregates made using pea proteins, to assemble formed fibrils into protein-based gels, and to study the rheological behavior of these gels. Micrometer-long fibrillar aggregates were observed after pea protein solutions had been heated for 20 h at pH 2.0. Following heating of pea proteins, it was observed that all of the proteins were hydrolyzed into peptides and that 50% of these peptides were assembled into fibrils. Changes on a structural level in pea proteins were studied using circular dichroism, transmission electron microscopy, and particle size analysis. During the fibril assembly process, an increase in aggregate size was observed, which coincided with an increase in thioflavin T binding, indicating the presence of β-sheet aggregates. Fibrils made using pea proteins were more branched and curly. Gel formation of preformed fibrils was induced by slow acidification from pH 7.0 to a final pH of around pH 5.0. The ability of pea protein-based fibrillar gels to fracture during an amplitude sweep was comparable to those of soy protein and whey protein-based fibrillar gels, although gels prepared from fibrils made using pea protein and soy protein were weaker than those of whey protein. The findings show that fibrils can be prepared from pea protein, which can be incorporated into protein-based fibrillar gels.
Original languageEnglish
Pages (from-to)2418-2427
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Early online date24 Feb 2014
DOIs
Publication statusPublished - 19 Mar 2014

Fingerprint

pea protein
Peas
Gels
gels
protein aggregates
Proteins
whey protein
soy protein
Soybean Proteins
peptides
circular dichroism spectroscopy
proteins
acidification
Peptides
transmission electron microscopy
particle size
Acidification
Circular Dichroism
Transmission Electron Microscopy
Particle Size

Keywords

  • fibril assembly
  • fibrillar aggregates
  • gel formation
  • pea protein
  • soy protein
  • whey protein

Cite this

Fibril formation from pea protein and subsequent gel formation. / Munialo, Claire Darizu; Martin, Anneke H.; Van Der Linden, Erik; De Jongh, Harmen H.J.

In: Journal of Agricultural and Food Chemistry, 19.03.2014, p. 2418-2427.

Research output: Contribution to journalArticle

Munialo, Claire Darizu ; Martin, Anneke H. ; Van Der Linden, Erik ; De Jongh, Harmen H.J. / Fibril formation from pea protein and subsequent gel formation. In: Journal of Agricultural and Food Chemistry. 2014 ; pp. 2418-2427.
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