Abstract
Objective: This study aims to investigate the association of Fc receptor-like 3 (FCRL3) gene variants with multiple sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD) in a Chinese population cohort.
Methods: In Stage 1, 154 MS patients, 109 NMOSD patients, and 301 normal controls were recruited, Sequenom MassARRAY technology was used for genotyping single nucleotide polymorphisms (SNPs). Stage 2 involved an independent cohort of 95 MS patients, 139 NMOSD patients, and 226 normal controls. Two FCRL3 SNPs (rs7528684 and rs11264799) were determined using allele-specific polymerase chain reaction (PCR) with specific primers.
Results: Allele C of rs7528684 emerged as a protective factor for MS. Allele A of rs11264799 exhibited no significant effect on MS or NMOSD. A notable disparity in rs7528684 genotype distribution was observed between oligoclonal band (OCB)-positive and OCB-negative MS patients. Allele C of rs7528684 exhibited an association with OCB-positive MS patients.
Conclusion: The findings suggest that the FCRL3 variant (rs7528684) is associated with MS rather than NMOSD. FCRL3 might significantly contribute to OCB synthesis, while the underlying mechanisms warrant further elucidation.
Methods: In Stage 1, 154 MS patients, 109 NMOSD patients, and 301 normal controls were recruited, Sequenom MassARRAY technology was used for genotyping single nucleotide polymorphisms (SNPs). Stage 2 involved an independent cohort of 95 MS patients, 139 NMOSD patients, and 226 normal controls. Two FCRL3 SNPs (rs7528684 and rs11264799) were determined using allele-specific polymerase chain reaction (PCR) with specific primers.
Results: Allele C of rs7528684 emerged as a protective factor for MS. Allele A of rs11264799 exhibited no significant effect on MS or NMOSD. A notable disparity in rs7528684 genotype distribution was observed between oligoclonal band (OCB)-positive and OCB-negative MS patients. Allele C of rs7528684 exhibited an association with OCB-positive MS patients.
Conclusion: The findings suggest that the FCRL3 variant (rs7528684) is associated with MS rather than NMOSD. FCRL3 might significantly contribute to OCB synthesis, while the underlying mechanisms warrant further elucidation.
| Original language | English |
|---|---|
| Article number | 1552149 |
| Number of pages | 9 |
| Journal | Frontiers in Neurology |
| Volume | 16 |
| DOIs | |
| Publication status | Published - 4 Jul 2025 |
Bibliographical note
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Funding
The author(s) declare that financial support was received for the research and/or publication of this article. This study was supported by the research foundation for distinguished scholar of Zhejiang University to Zhi-Ying Wu (188020\u2013193810101/089) and the National Natural Science Foundation of China (82201513).
| Funders | Funder number |
|---|---|
| Zhejiang University | 188020–193810101/089 |
| Zhejiang University | |
| National Natural Science Foundation of China | 82201513 |
| National Natural Science Foundation of China |
Keywords
- multiple sclerosis
- neuromyelitis optica spectrum disorders
- FCRL3
- variant
- association