Evolutionary conserved cysteines function as cis-acting regulators of Arabidopsis PIN-FORMED 2 distribution

Katarzyna Retzer, Jozef Lacek, Roman Skokan, Charo del Genio, Stanislav Vosolsobě, Martina Laňková, Kateřina Malínská, Nataliia Konstantinova, Eva Zažímalová, Richard Napier, Jan Petrášek, Christian Luschnig

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Abstract

Coordination of plant development requires modulation of growth responses that are under control of the phytohormone auxin. PIN-FORMED plasma membrane proteins, involved in intercellular transport of the growth regulator, are key to the transmission of such auxin signals and subject to multilevel surveillance mechanisms, including reversible post-translational modifications. Apart from well-studied PIN protein modifications, namely phosphorylation and ubiquitylation, no further post-translational modifications have been described so far. Here, we focused on root-specific Arabidopsis PIN2 and explored functional implications of two evolutionary conserved cysteines, by a combination of in silico and molecular approaches. PIN2 sequence alignments and modeling predictions indicated that both cysteines are facing the cytoplasm and therefore would be accessible to redox status-controlled modifications. Notably, mutant pin2C−A alleles retained functionality, demonstrated by their ability to almost completely rescue defects of a pin2 null allele, whereas high resolution analysis of pin2C−A localization revealed increased intracellular accumulation, and altered protein distribution within plasma membrane micro-domains. The observed effects of cysteine replacements on root growth and PIN2 localization are consistent with a model in which redox status-dependent cysteine modifications participate in the regulation of PIN2 mobility, thereby fine-tuning polar auxin transport.
Original languageEnglish
Article number2274
JournalInternational Journal of Molecular Sciences
Volume18
Issue number11
DOIs
Publication statusPublished - 29 Oct 2017

Bibliographical note

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.

Keywords

  • Auxin
  • PIN proteins
  • Plasma membrane protein sorting
  • Protein mobility
  • Intracellular distribution
  • Root phenotype
  • Arabidopsis
  • Protein modeling
  • SRRF

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