Enrichment of rat NK cytotoxicity for H2-negative murine embryonal carcinoma cells by panning and short-term culture in TCGF

S. M. Bell, R. Aspinall, P. L. Stern

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The rat natural killer (NK) cell, defined here by spontaneous lysis of H2-negative embryonal carcinoma (EC) cells, was investigated with respect to its antigenic phenotype. Rat spleen cells were separated by panning into adherent and non-adherent populations after incubation with monoclonal antibodies defining differentiation antigens of rat lymphoid cells. This method achieved considerable NK enrichment in some of the panned populations (for example, W3/13 non-adherent cells, up to 20-fold), but emphasized the heterogeneous surface antigen expression of the NK cell type. These enrichment procedures were used to establish bulk cultures of rat NK cells which increased in specific activity over several weeks when grown in T cell-growth factor (TCGF) and could be cloned in soft agarose.

Original languageEnglish
Pages (from-to)23-32
Number of pages10
JournalImmunology
Volume53
Issue number1
Publication statusPublished - 1984
Externally publishedYes

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Embryonal Carcinoma Stem Cells
Interleukin-2
Natural Killer Cells
Differentiation Antigens
Surface Antigens
Sepharose
Population
Spleen
Monoclonal Antibodies
Lymphocytes
Phenotype

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Enrichment of rat NK cytotoxicity for H2-negative murine embryonal carcinoma cells by panning and short-term culture in TCGF. / Bell, S. M.; Aspinall, R.; Stern, P. L.

In: Immunology, Vol. 53, No. 1, 1984, p. 23-32.

Research output: Contribution to journalArticle

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