Efficacy of a novel neem oil formulation (RP03™) to control the poultry red mite Dermanyssus gallinae

A Camarda, N Pugliese, A Bevilacqua, E Circella, L Gradoni, D George, O Sparagano, A Giangaspero

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20 Citations (Scopus)
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Dermanyssus gallinae (Mesostigmata: Dermanyssidae) is the most harmful ectoparasite of laying hens, represents an occupational hazard for poultry workers, and a growing threat to medical science per se. There is increasing demand for alternative products, including plant-derived acaricides, with which to control the mite. The present study investigated the efficacy of neem oil against D. gallinae on a heavily infested commercial laying hen farm. A novel formulation of 20% neem oil, diluted from a 2400-p.p.m. azadirachtin-concentrated stock (RP03™), was administered by nebulization three times in 1 week. Using corrugated cardboard traps, mite density was monitored before, during and after treatment and results were statistically analysed. Mite populations in the treated block showed 94.65%, 99.64% and 99.80% reductions after the first, second and third product administrations, respectively. The rate of reduction of the mite population was significantly higher in the treated block (P < 0.001) compared with the control and buffer blocks. The results suggest the strong bioactivity of neem, and specifically of the patented neem-based formulation RP03™, against D. gallinae. The treatment was most effective in the 10 days following the first application and its effects persisted for over 2 months. Further studies will aim to overcome observed side effects of treatment represented by an oily layer on equipment and eggs.

Original languageEnglish
Pages (from-to)290-297
JournalMedical and Veterinary Entomology
Issue number3
Early online date8 Feb 2018
Publication statusPublished - Sept 2018


  • Azadirachta indica
  • Dermanyssus gallinae
  • acaricide
  • enriched colony system
  • laying hens
  • neem
  • zoonosis


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