Effects of CO2 laser irradiation on the surface properties of magnesia-partially stabilised zirconia (MgO-PSZ) bioceramic and the subsequent improvements in human osteoblast cell adhesion

L. Hao, J. Lawrence, K. S. Chian

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In order to acquire the surface properties favouring osseointegration at the implant and bone interface, human foetal osteoblast cells (hFOB) were used in an in vitro test to examine changes in cell adhesion on a magnesia-partially, stabilised zirconia (MgO-PSZ) bioceramic after CO 2 laser treatment. The surface roughness, microstructure, crystal size and surface energy of untreated and CO 2 laser-treated MgO-PSZ were fully characterised. The in vitro cell evaluation revealed a more favourable cell response on the CO2 laser-treated MgO-PSZ than on the untreated sample. After 24-h cell incubation, no cell was observed on the MgO-PSZ, whereas a few cells attached on the CO2 laser-treated MgO-PSZ and showed well spread and good attachment. Moreover, the cell coverage density indicating, cell proliferation generally increases with CO2 laser power densities applied in the experiments. The enhancement of the surface energy of the MgO-PSZ, especially its polar component caused by the CO2 laser treatment, was found to play a significant role in the initial cell attaching, thus enhancing the cell growth. Moreover, the change in topography induced by the CO2 laser treatment was identified as one of the factors influencing the hFOB cell response.

Original languageEnglish
Pages (from-to)81-105
Number of pages25
JournalJournal of Biomaterials Applications
Volume19
Issue number2
DOIs
Publication statusPublished - 1 Oct 2004
Externally publishedYes

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Magnesium Oxide
Bioceramics
Osteoblasts
Cell adhesion
Magnesia
Laser beam effects
Zirconia
Surface properties
Lasers
Carbon Monoxide
Interfacial energy
Crystal microstructure
Cell proliferation
Cell growth
zirconium oxide
Topography
Bone
Surface roughness

Keywords

  • CO laser
  • Human osteoblast cell adhesion
  • Magnesia-partially stahilised zirconia (MgO-PSZ)
  • Surface energy
  • Topography

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

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abstract = "In order to acquire the surface properties favouring osseointegration at the implant and bone interface, human foetal osteoblast cells (hFOB) were used in an in vitro test to examine changes in cell adhesion on a magnesia-partially, stabilised zirconia (MgO-PSZ) bioceramic after CO 2 laser treatment. The surface roughness, microstructure, crystal size and surface energy of untreated and CO 2 laser-treated MgO-PSZ were fully characterised. The in vitro cell evaluation revealed a more favourable cell response on the CO2 laser-treated MgO-PSZ than on the untreated sample. After 24-h cell incubation, no cell was observed on the MgO-PSZ, whereas a few cells attached on the CO2 laser-treated MgO-PSZ and showed well spread and good attachment. Moreover, the cell coverage density indicating, cell proliferation generally increases with CO2 laser power densities applied in the experiments. The enhancement of the surface energy of the MgO-PSZ, especially its polar component caused by the CO2 laser treatment, was found to play a significant role in the initial cell attaching, thus enhancing the cell growth. Moreover, the change in topography induced by the CO2 laser treatment was identified as one of the factors influencing the hFOB cell response.",
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AB - In order to acquire the surface properties favouring osseointegration at the implant and bone interface, human foetal osteoblast cells (hFOB) were used in an in vitro test to examine changes in cell adhesion on a magnesia-partially, stabilised zirconia (MgO-PSZ) bioceramic after CO 2 laser treatment. The surface roughness, microstructure, crystal size and surface energy of untreated and CO 2 laser-treated MgO-PSZ were fully characterised. The in vitro cell evaluation revealed a more favourable cell response on the CO2 laser-treated MgO-PSZ than on the untreated sample. After 24-h cell incubation, no cell was observed on the MgO-PSZ, whereas a few cells attached on the CO2 laser-treated MgO-PSZ and showed well spread and good attachment. Moreover, the cell coverage density indicating, cell proliferation generally increases with CO2 laser power densities applied in the experiments. The enhancement of the surface energy of the MgO-PSZ, especially its polar component caused by the CO2 laser treatment, was found to play a significant role in the initial cell attaching, thus enhancing the cell growth. Moreover, the change in topography induced by the CO2 laser treatment was identified as one of the factors influencing the hFOB cell response.

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