This study determined the effectiveness of antioxidant supplementation on high-intensity exercise-heat stress. Six males completed a high-intensity running protocol twice in temperate conditions (TEMP; 20.4°C), and twice in hot conditions (HOT; 34.7°C). Trials were completed following7 days supplementation with 70 ml·day−1 effective microorganism-X (EM-X; TEMPEMX or HOTEMX) or placebo (TEMPPLA or HOTPLA). Plasma extracellular Hsp72 (eHsp72) and superoxide dismutase (SOD) were measured by ELISA. eHsp72 and SOD increased pre-post exercise (p <0.001), with greater eHsp72 (p <0.001) increases observed in HOT (+1.5 ng·ml−1) compared to TEMP (+0.8 ng·ml−1). EM-X did not influence eHsp72 (p > 0.05). Greater (p <0.001) SOD increases were observed in HOT (+0.22 U·ml−1) versus TEMP (+0.10 U·ml−1) with SOD reduced in HOTEMX versus HOTPLA (p = 0.001). Physiological and perceptual responses were all greater (p <0.001) in HOT versus TEMP conditions, with no difference followed EM-X (p > 0.05). EM-X supplementation attenuated the SOD increases following HOT, potentiating its application as an ergogenic aid to ameliorate oxidative stress.
|Journal||Research in Sports Medicine: An International Journal|
|Early online date||31 Mar 2016|
|Publication status||Published - 2016|
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- heat shock proteins (HSP)
- oxidative stress
- redox balance
- repeated sprint