Effect of laser treatment on the attachment and viability of mesenchymal stem cell responses on shape memory NiTi alloy

C. W. Chan, I. Hussain, D. G. Waugh, J. Lawrence, H. C. Man

Research output: Contribution to journalArticle

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Abstract

The objectives of this study were to investigate the effect of laser-induced surface features on the morphology, attachment and viability of mesenchymal stem cells (MSCs) at different periods of time, and to evaluate the biocompatibility of different zones: laser-melted zone (MZ), heat-affected zone (HAZ) and base metal (BM) in laser-treated NiTi alloy. The surface morphology and composition were studied by scanning electron microscope (SEM) and X-ray photoemission spectroscopy (XPS), respectively. The cell morphology was examined by SEM while the cell counting and viability measurements were done by hemocytometer and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The results indicated that the laser-induced surface features, such as surface roughening, presence of anisotropic dendritic pattern and complete surface Ni oxidation were beneficial to improve the biocompatibility of NiTi as evidenced by the highest cell attachment (4 days of culture) and viability (7 days of culture) found in the MZ. The biocompatibility of the MZ was the best, followed by the BM with the HAZ being the worst. The defective and porous oxide layer as well as the coarse grained structure might attribute to the inferior cell attachment (4 days of culture) and viability (7 days of culture) on the HAZ compared with the BM which has similar surface morphology.

Original languageEnglish
Pages (from-to)254-263
Number of pages10
JournalMaterials Science and Engineering C
Volume42
DOIs
Publication statusPublished - 1 Sep 2014
Externally publishedYes

Fingerprint

stem cells
shape memory alloys
Stem cells
Mesenchymal Stromal Cells
Shape memory effect
viability
attachment
Lasers
Heat affected zone
Biocompatibility
heat affected zone
Hot Temperature
Metals
biocompatibility
lasers
Surface morphology
Electron microscopes
Electrons
cells
Scanning

Keywords

  • Fiber laser
  • Laser treatment
  • Mesenchymal stem cell (MSC)
  • NiTi
  • Shape memory alloy (SMA)

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials
  • Medicine(all)

Cite this

Effect of laser treatment on the attachment and viability of mesenchymal stem cell responses on shape memory NiTi alloy. / Chan, C. W.; Hussain, I.; Waugh, D. G.; Lawrence, J.; Man, H. C.

In: Materials Science and Engineering C, Vol. 42, 01.09.2014, p. 254-263.

Research output: Contribution to journalArticle

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AU - Man, H. C.

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