Naleh fish Barbonymus sp. is a commercial freshwater fish, which is indigenous to Aceh, Indonesia. The population of this species has declined over the years as a result of habitat perturbations and overfishing. Hence, the crucial need to develop a cryopreservation method to support breeding programs. This involved the use of a cryoprotectant as an important component. The objective of this study, therefore, was to explore the best cryoprotectant for naleh fish spermatozoa, and a total of five types were tested. These include the DMSO, Methanol, Ethanol, Glycerol, and Ethylene Glycol at a similar concentration of 10%, which were individually combined with 15% egg yolk, and every treatment was performed in three replications. Conversely, Ringer’s solution was adopted as an extender, and the sperm was cryopreserved in liquid nitrogen for 15 days. The results showed significant influence on sperm motility and viability, as well as egg fertility of naleh fish (P <0.05), although the DMSO provided the best outcome, compared to others at 47.17%, 50.13%, and 45.67%, respectively. Furthermore, DNA fragmentation had not occurred in the fresh and cryopreserved sperm samples, indicating the protective effect of tested cryoprotectants. It is concluded that the 10% DMSO and 15% egg yolk is the best cryoprotectant for naleh fish spermatozoa.
|Translated title of the contribution||Effect of cryoprotectant on the motility, viability, fertilization, and DNA integrity of naleh fish Barbonymus sp. (Cyprinidae) sperm|
|Journal||Brazilian Journal of Veterinary Research and Animal Science|
|Publication status||Published - 16 Jul 2021|
Bibliographical noteFunding Information:
This study was supported by the Syiah Kuala University through the Professorship scheme 2019. Therefore, the author thanks the Rector of Syiah Kuala University for supporting this study. The assistance of all members of Ichthyos Research Group and Mr. Khaidir from Blang Adei Jaya Hatchery is also acknowledged.
© 2021, Universidade de Sao Paulo. Faculdade de Medicina Veterinaria e Zootecnia. All rights reserved.
- DNA fragmentation
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