Dose and latency effects of leucine supplementation in modulating glucose homeostasis: opposite effects in healthy and glucocorticoid-induced insulin-resistance states

Nelo Eidy Zanchi, Lucas Guimarães-Ferreira, Mário Alves de Siqueira-Filho, Vitor Felitti, Humberto Nicastro, Carlos Bueno, Fábio Santos Lira, Marshall Alan Naimo, Patrícia Campos-Ferraz, Maria Tereza Nunes, Marília Seelaender, Carla Roberta de Oliveira Carvalho, François Blachier, Antonio Herbert Lancha

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)
14 Downloads (Pure)

Abstract

Dexamethasone (DEXA) is a potent immunosupressant and anti-inflammatory agent whose main side effects are muscle atrophy and insulin resistance in skeletal muscles. In this context, leucine supplementation may represent a way to limit the DEXA side effects. In this study, we have investigated the effects of a low and a high dose of leucine supplementation (via a bolus) on glucose homeostasis, muscle mass and muscle strength in energy-restricted and DEXA-treated rats. Since the leucine response may also be linked to the administration of this amino acid, we performed a second set of experiments with leucine given in bolus (via gavage) versus leucine given via drinking water. Leucine supplementation was found to produce positive effects (e.g., reduced insulin levels) only when administrated in low dosage, both via the bolus or via drinking water. However, under DEXA treatment, leucine administration was found to significantly influence this response, since leucine supplementation via drinking water clearly induced a diabetic state, whereas the same effect was not observed when supplied via the gavage.

Original languageEnglish
Pages (from-to)1851-1867
Number of pages17
JournalNutrients
Volume4
Issue number12
DOIs
Publication statusPublished - 27 Nov 2012
Externally publishedYes

Bibliographical note

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license
(http://creativecommons.org/licenses/by/3.0/).

Keywords

  • Animals
  • Blood Glucose/metabolism
  • Dexamethasone/adverse effects
  • Dietary Supplements
  • Dose-Response Relationship, Drug
  • Drug Administration Routes
  • Glucocorticoids/adverse effects
  • Homeostasis/drug effects
  • Insulin/blood
  • Insulin Resistance
  • Leucine/administration & dosage
  • Male
  • Muscle Strength/drug effects
  • Muscle, Skeletal/drug effects
  • Muscular Atrophy/chemically induced
  • Rats
  • Rats, Wistar

Fingerprint

Dive into the research topics of 'Dose and latency effects of leucine supplementation in modulating glucose homeostasis: opposite effects in healthy and glucocorticoid-induced insulin-resistance states'. Together they form a unique fingerprint.

Cite this