Does Dietary-Induced Obesity in Old Age Impair the Contractile Performance of Isolated Mouse Soleus, Extensor Digitorum Longus and Diaphragm Skeletal Muscles?

Cameron Hill, Rob James, Valerie Cox, Jason Tallis

Research output: Contribution to journalArticle

2 Citations (Scopus)
15 Downloads (Pure)

Abstract

Ageing and obesity independently have been shown to significantly impair isolated muscle contractile properties, though their synergistic effects are poorly understood. We uniquely examined the effects of 9 weeks of a high-fat diet (HFD) on isometric force, work loop power output (PO) across a range of contractile velocities, and fatigability of 79-week-old soleus, extensor digitorum longus (EDL) and diaphragm compared with age-matched lean controls. The dietary intervention resulted in a significant increase in body mass and gonadal fat pad mass compared to the control group. Despite increased muscle mass for HFD soleus and EDL, absolute isometric force, isometric stress (force/CSA), PO normalised to muscle mass and fatigability was unchanged, although absolute PO was significantly greater. Obesity did not cause an alteration in the contractile velocity that elicited maximal PO. In the obese group, normalised diaphragm PO was significantly reduced, with a tendency for reduced isometric stress and fatigability was unchanged. HFD soleus isolated from larger animals produced lower maximal PO which may relate to impaired balance in older, larger adults. The increase in absolute PO is smaller than the magnitude of weight gain, meaning in vivo locomotor function is likely to be impaired in old obese adults, with an association between greater body mass and poorer normalised power output for the soleus. An obesity-induced reduction in diaphragm contractility will likely impair in vivo respiratory function and consequently contribute further to the negative cycle of obesity.
Original languageEnglish
Article number505
Number of pages18
JournalNutrients
Volume11
Issue number3
DOIs
Publication statusPublished - 27 Feb 2019

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diaphragm
Diaphragm
skeletal muscle
Skeletal Muscle
High Fat Diet
obesity
Obesity
high fat diet
mice
Muscles
muscles
labor force
Weight Gain
lung function
Adipose Tissue
Control Groups
weight gain
lipids
animals

Bibliographical note

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Copyright © and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders.

Keywords

  • Ageing
  • Muscle
  • Obesity
  • Power
  • Work loop

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Does Dietary-Induced Obesity in Old Age Impair the Contractile Performance of Isolated Mouse Soleus, Extensor Digitorum Longus and Diaphragm Skeletal Muscles? / Hill, Cameron; James, Rob; Cox, Valerie; Tallis, Jason.

In: Nutrients, Vol. 11, No. 3, 505, 27.02.2019.

Research output: Contribution to journalArticle

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