Do plants contain G protein-coupled receptors?

Bruck Taddese, Graham J.G. Upton, Gregory R. Bailey, Siân R.D. Jordan, Nuradin Y. Abdulla, Philip J. Reeves, Christopher A. Reynolds

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)


Whether G protein-coupled receptors (GPCRs) exist in plants is a fundamental biological question. Interest in deorphanizing new GPCRs arises because of their importance in signaling. Within plants, this is controversial, as genome analysis has identified 56 putative GPCRs, includingGprotein-coupled receptor1 (GCR1), which is reportedly a remote homolog to class A, B, and E GPCRs. Of these, GCR2 is not a GPCR; more recently, it has been proposed that none are, not even GCR1. We have addressed this disparity between genome analysis and biological evidence through a structural bioinformatics study, involving fold recognition methods, from which only GCR1 emerges as a strong candidate. To further probe GCR1, we have developed a novel helix-alignment method, which has been benchmarked against the class A-class B-class F GPCR alignments. In addition, we have presented a mutually consistent set of alignments ofGCR1 homologs to class A, class B, and class FGPCRs and shownthatGCR1 is closer to classAand/or class B GPCRs than class A, class B, or class F GPCRs are to each other. To further probe GCR1, we have aligned transmembrane helix 3 of GCR1 to each of the six GPCR classes. Variability comparisons provide additional evidence that GCR1 homologs have the GPCR fold. From the alignments and a GCR1 comparative model, we have identified motifs that are common to GCR1, class A, B, and E GPCRs. We discuss the possibilities that emerge from this controversial evidence that GCR1 has a GPCR fold.

Original languageEnglish
Pages (from-to)287-307
Number of pages21
JournalPlant Physiology
Issue number1
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Bibliographical note

This article can be viewed online without a subscription.

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science


Dive into the research topics of 'Do plants contain G protein-coupled receptors?'. Together they form a unique fingerprint.

Cite this