Diversity of Coxiella-like and Francisella-like endosymbionts, and Rickettsia spp., Coxiella burnetii as pathogens in the tick populations of Slovakia, Central Europe

Eva Špitalská, Olivier Sparagano, Michal Stanko, Katarína Schwarzová, Zdenko Špitalský, Ľudovít Škultéty, Sabína Fumačová Havlíková

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Abstract

Ticks are important vectors of pathogens affecting humans and animals worldwide. They do not only carry pathogens but diverse commensal and symbiotic microorganisms are also present in ticks. A molecular screening for tick-borne pathogens and endosymbionts was carried out in Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis inermis questing ticks collected in Slovakia. The presence of Rickettsia spp., Coxiella burnetii, Coxiella-like and Francisella-like microorganisms was evaluated by PCR in 605 individuals and by randomly sequencing 66 samples. Four species of rickettsiae (R. raoultii, R. slovaca, R. helvetica and R. monacensis) were identified and reported with an overall prevalence range between 0.4 and 50.3% (±8.0) depending on tick species, sex and locality. Partial sequencing of the gltA gene of 5 chosen samples in H. inermis showed 99% identity with Candidatus Rickettsia hungarica. The total prevalence of C. burnetii in ticks was 2.2 ± 1.7%; bacteria were confirmed in I. ricinus and D. reticulatus ticks. The sequences from 2 D. reticulatus males and 1 I. ricinus female ticks were compared to GenBank submissions and a 99.8% match was obtained with the pathogenic C. burnetii. Coxiella–like endosymbionts were registered in all three species of ticks from all studied sites with an average prevalence of 32.7 ± 3.7%. A phylogenetic analysis of this Coxiella sp. showed that it does not group with the pathogenic C. burnetii. The prevalence of Francisella-like microorganisms in questing ticks was 47.9 ± 3.9%, however H. inermis (n = 108) were not infested. Obtained sequences were 98% identical with previously identified Francisella-like endosymbionts in D. reticulatus and I. ricinus. Coxiella-like and Francisella-like microorganisms were identified for the first time in Slovakia, they might be considered as a non-pathogenic endosymbiont of I. ricinus, D. reticulatus and H. inermis, and future investigations could aim to assess their role in these ticks. However, this work provided further data and broadened our knowledge on bacterial pathogens and endosymbionts present in ticks in Slovakia to help understanding co-infestations, combined treatments and public health issues linked to tick bites.

Original languageEnglish
Pages (from-to)1207-1211
Number of pages5
JournalTicks and Tick-borne Diseases
Volume9
Issue number5
Early online date3 May 2018
DOIs
Publication statusPublished - Jul 2018

Fingerprint

Francisella
Coxiella
Coxiella burnetii
Rickettsia
Slovakia
endosymbionts
Ticks
Central European region
ticks
Dermacentor reticulatus
pathogens
Ixodes
Ixodes ricinus
Population
microorganisms
Dermacentor
tick bites
Tick Bites
Haemaphysalis
microsymbionts

Bibliographical note

NOTICE: this is the author’s version of a work that was accepted for publication in Ticks and Tick-borne Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Ticks and Tick-borne Diseases, (2018)
DOI: 10.1016/j.ttbdis.2018.05.002
© 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

  • Endosymbiont
  • Pathogen
  • Slovakia
  • Tick

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Insect Science
  • Infectious Diseases

Cite this

Diversity of Coxiella-like and Francisella-like endosymbionts, and Rickettsia spp., Coxiella burnetii as pathogens in the tick populations of Slovakia, Central Europe. / Špitalská, Eva; Sparagano, Olivier; Stanko, Michal; Schwarzová, Katarína; Špitalský, Zdenko; Škultéty, Ľudovít; Havlíková, Sabína Fumačová.

In: Ticks and Tick-borne Diseases, Vol. 9, No. 5, 07.2018, p. 1207-1211.

Research output: Contribution to journalArticle

Špitalská, Eva ; Sparagano, Olivier ; Stanko, Michal ; Schwarzová, Katarína ; Špitalský, Zdenko ; Škultéty, Ľudovít ; Havlíková, Sabína Fumačová. / Diversity of Coxiella-like and Francisella-like endosymbionts, and Rickettsia spp., Coxiella burnetii as pathogens in the tick populations of Slovakia, Central Europe. In: Ticks and Tick-borne Diseases. 2018 ; Vol. 9, No. 5. pp. 1207-1211.
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