Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Eva Spitalska; Alessandra Torina; Vincenza Cannella; Santo Caracappa; Olivier A E Sparagano

doi:10.1007/s00436-004-1217-2

Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Eva Spitalska, Alessandra Torina, Vincenza Cannella, Santo Caracappa, Olivier A E Sparagano

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

Original language	English
Pages (from-to)	318-320
Number of pages	3
Journal	Parasitology Research
Volume	94
Issue number	4
DOIs	https://doi.org/10.1007/s00436-004-1217-2
Publication status	Published - 1 Oct 2004
Externally published	Yes

Funding

Acknowledgements This study was partially supported by a NATO/ Royal Society Postdoctoral Fellowship awarded to Dr. E. Spit-alska. The experiments presented in this paper comply with the current UK laws.

Keywords

Restriction Fragment Length Polymorphism
Mixed Infection
Small Ruminant
Babesia
Ixodid Tick

ASJC Scopus subject areas

Parasitology

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10.1007/s00436-004-1217-2

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title = "Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene",

abstract = "Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.",

keywords = "Restriction Fragment Length Polymorphism , Mixed Infection , Small Ruminant , Babesia , Ixodid Tick",

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AU - Spitalska, Eva

AU - Torina, Alessandra

AU - Cannella, Vincenza

AU - Caracappa, Santo

AU - Sparagano, Olivier A E

PY - 2004/10/1

Y1 - 2004/10/1

N2 - Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

AB - Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

KW - Restriction Fragment Length Polymorphism

KW - Mixed Infection

KW - Small Ruminant

KW - Babesia

KW - Ixodid Tick

UR - https://www.scopus.com/pages/publications/8744251033

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DO - 10.1007/s00436-004-1217-2

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JO - Parasitology Research

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IS - 4

ER -

Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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