Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Eva Spitalska, Alessandra Torina, Vincenza Cannella, Santo Caracappa, Olivier A E Sparagano

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

Original languageEnglish
Pages (from-to)318-320
Number of pages3
JournalParasitology Research
Volume94
Issue number4
DOIs
Publication statusPublished - 1 Oct 2004
Externally publishedYes

Keywords

  • Restriction Fragment Length Polymorphism
  • Mixed Infection
  • Small Ruminant
  • Babesia
  • Ixodid Tick

ASJC Scopus subject areas

  • Parasitology

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