Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Eva Spitalska; Alessandra Torina; Vincenza Cannella; Santo Caracappa; Olivier A E Sparagano

doi:10.1007/s00436-004-1217-2

Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Eva Spitalska, Alessandra Torina, Vincenza Cannella, Santo Caracappa, Olivier A E Sparagano

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

Original language	English
Pages (from-to)	318-320
Number of pages	3
Journal	Parasitology Research
Volume	94
Issue number	4
DOIs	https://doi.org/10.1007/s00436-004-1217-2
Publication status	Published - 1 Oct 2004
Externally published	Yes

Keywords

Restriction Fragment Length Polymorphism
Mixed Infection
Small Ruminant
Babesia
Ixodid Tick

ASJC Scopus subject areas

Parasitology

Access to Document

10.1007/s00436-004-1217-2

Cite this

@article{7dcc8976f31a4a7fb3b838e3d1a653c1,

title = "Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene",

abstract = "Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.",

keywords = "Restriction Fragment Length Polymorphism , Mixed Infection , Small Ruminant , Babesia , Ixodid Tick",

author = "Eva Spitalska and Alessandra Torina and Vincenza Cannella and Santo Caracappa and Sparagano, {Olivier A E}",

year = "2004",

month = oct,

day = "1",

doi = "10.1007/s00436-004-1217-2",

language = "English",

volume = "94",

pages = "318--320",

journal = "Parasitology Research",

issn = "0932-0113",

publisher = "Springer Verlag",

number = "4",

}

TY - JOUR

T1 - Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

AU - Spitalska, Eva

AU - Torina, Alessandra

AU - Cannella, Vincenza

AU - Caracappa, Santo

AU - Sparagano, Olivier A E

PY - 2004/10/1

Y1 - 2004/10/1

N2 - Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

AB - Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

KW - Restriction Fragment Length Polymorphism

KW - Mixed Infection

KW - Small Ruminant

KW - Babesia

KW - Ixodid Tick

UR - http://www.scopus.com/inward/record.url?scp=8744251033&partnerID=8YFLogxK

U2 - 10.1007/s00436-004-1217-2

DO - 10.1007/s00436-004-1217-2

M3 - Article

C2 - 15372232

AN - SCOPUS:8744251033

SN - 0932-0113

VL - 94

SP - 318

EP - 320

JO - Parasitology Research

JF - Parasitology Research

IS - 4

ER -

Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this