Differential expression of cold- and diet-specific genes encoding two carp liver Δ9-acyl-CoA desaturase isoforms

S.D. Polley, P.E. Tiku, R.T. Trueman, M.X. Caddick, I.Y. Morozov, A.R. Cossins

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

Carp respond to cold by the upregulated expression of Δ9-acyl-CoA desaturase. Here we report the cloning and characterization of Cds2, a second Δ9-acyl CoA-desaturase expressed in carp liver. Both Cds1and Cds2 complemented the ole1 mutation inSaccharomyces cerevisiae, permitting the synthesis of Δ9-monounsaturates, confirming their identity as Δ9-desaturases. We demonstrate that under a standard feeding regime it is theCds2, and not Cds1, transcript that is transiently upregulated during the first few days of cooling from 30°C to 10°C, the period when cold-induced membrane restructuring occurs. Cds2 exists as two differentially spliced transcripts, differing by a small segment from the 3′-untranslated region, the ratio of which varies with temperature. Feeding a diet enriched in saturated fats produced a fourfold increase inCds1 transcript levels, which was blocked by cooling to 15°C. Cds2 transcript levels, however, showed no substantial response to the saturated diet. Thus carp liver uniquely expresses two isoforms of Δ9-acyl CoA desaturase, possibly formed by a recent duplication event, that are differentially regulated by cooling and dietary treatment.
Original languageEnglish
Pages (from-to)41-50
Number of pages10
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume284
Issue number1
DOIs
Publication statusPublished - 1 Jan 2003
Externally publishedYes

Keywords

  • temperature adaptation
  • lipid adaption
  • membrane adaption
  • homeoviscous adaptation

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