Diet, lifestyle and risk of K-ras mutation-positive and -negative colorectal adenomas

Petra A. Wark, Wieke Van Der Kuil, Janneke Ploemacher, Goos N P Van Muijen, Chris J J Mulder, Matty P. Weijenberg, Frans J. Kok, Ellen Kampman

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    K-ras mutation-positive (K-ras+) and -negative (K-ras -) colorectal adenomas may differ clinically and pathologically. As environmental compounds may cause mutations in the growth-related K-ras oncogene or affect clonal selection depending on mutational status, we evaluated whether the aetiology of K-ras+ and K-ras- adenomas differs. K-ras mutations in codons 12 and 13 were assessed in colorectal adenoma tissue (K-ras+: n = 81, K-ras-: n = 453). Dietary and lifestyle data were collected through questionnaires that were also administered to 709 polyp-free controls. Multiple logistic regression analyses showed that intake of vitamin B2 and monounsaturated fat were differently associated with risk of K-ras+ and K-ras- adenomas; vitamin B2 was inversely associated with K-ras- (highest vs. lowest tertile: odds ratio (OR) = 0.70, 95% confidence interval (CI) = 0.50-0.97, p trend = 0.020), but not with K-ras+ adenomas, and a positive association with monounsaturated fat was confined to K-ras- adenomas (OR = 1.57, 95% CI = 1.06-2.34, p trend = 0.029). Besides, potential, not statistically significant, differences in risk arose because red meat was distinctly positively associated with K-ras+ adenomas (OR = 1.70, 95% CI = 0.94-3.09, p trend = 0.061); total dietary and polyunsaturated fat tended to be inversely associated with risk of K-ras+ but not of K-ras- adenomas; inverse associations with dairy products, calcium, protein and tea were confined to K-ras- adenomas, and smoking was more markedly positively associated with K-ras- adenomas. No differences in risk of K-ras and K-ras - adenomas could be detected for other factors. In conclusion, dietary and lifestyle factors may influence risk of K-ras+ and K-ras- adenomas differently. However, epidemiological literature on diet, lifestyle and colorectal K-ras mutations is inconsistent.

    Original languageEnglish
    Pages (from-to)398-405
    Number of pages8
    JournalInternational Journal of Cancer
    Issue number2
    Early online date28 Feb 2006
    Publication statusPublished - 15 Jul 2006


    • Colorectal adenomatous polyps
    • Diet
    • K-ras
    • Lifestyle
    • Molecular epidemiology

    ASJC Scopus subject areas

    • Cancer Research
    • Oncology

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    Wark, P. A., Van Der Kuil, W., Ploemacher, J., Van Muijen, G. N. P., Mulder, C. J. J., Weijenberg, M. P., ... Kampman, E. (2006). Diet, lifestyle and risk of K-ras mutation-positive and -negative colorectal adenomas. International Journal of Cancer, 119(2), 398-405.