Development of specific oligonucleotide probes to detect Vibrio species: A preliminary study

Umberto Molini; Narut Thanantong; Annunziata Giangaspero; Olivier A.E. Sparagano

doi:10.1196/annals.1428.049

Development of specific oligonucleotide probes to detect Vibrio species: A preliminary study

Umberto Molini
, Narut Thanantong
, Annunziata Giangaspero
, Olivier A.E. Sparagano

Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise Giuseppe Caporale
Newcastle University
University of Foggia

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Citations (Scopus)

Abstract

Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.

Original language	English
Title of host publication	Animal Biodiversity and Emerging Diseases Prediction and Prevention
Publisher	Wiley
Pages	312-314
Number of pages	3
Volume	1149
ISBN (Print)	9781573317146
DOIs	https://doi.org/10.1196/annals.1428.049
Publication status	Published - 15 Dec 2008
Externally published	Yes

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1149
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 14 Life Below Water

Keywords

Diagnostic
PCR
Reverse line blot
Vibrio

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
History and Philosophy of Science

Access to Document

10.1196/annals.1428.049

Cite this

Development of specific oligonucleotide probes to detect Vibrio species: A preliminary study. / Molini, Umberto; Thanantong, Narut; Giangaspero, Annunziata et al.
Animal Biodiversity and Emerging Diseases Prediction and Prevention. Vol. 1149 Wiley, 2008. p. 312-314 (Annals of the New York Academy of Sciences; Vol. 1149).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

@inbook{2b79cae3ee7640c2bcfaf4f74ce4b98d,

title = "Development of specific oligonucleotide probes to detect Vibrio species: A preliminary study",

abstract = "Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.",

keywords = "Diagnostic, PCR, Reverse line blot, Vibrio",

author = "Umberto Molini and Narut Thanantong and Annunziata Giangaspero and Sparagano, \{Olivier A.E.\}",

year = "2008",

month = dec,

day = "15",

doi = "10.1196/annals.1428.049",

language = "English",

isbn = "9781573317146",

volume = "1149",

series = "Annals of the New York Academy of Sciences",

publisher = "Wiley",

pages = "312--314",

booktitle = "Animal Biodiversity and Emerging Diseases Prediction and Prevention",

address = "Germany",

}

TY - CHAP

T1 - Development of specific oligonucleotide probes to detect Vibrio species

T2 - A preliminary study

AU - Molini, Umberto

AU - Thanantong, Narut

AU - Giangaspero, Annunziata

AU - Sparagano, Olivier A.E.

PY - 2008/12/15

Y1 - 2008/12/15

N2 - Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.

AB - Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.

KW - Diagnostic

KW - PCR

KW - Reverse line blot

KW - Vibrio

UR - https://www.scopus.com/pages/publications/57849098915

U2 - 10.1196/annals.1428.049

DO - 10.1196/annals.1428.049

M3 - Chapter

C2 - 19120237

AN - SCOPUS:57849098915

SN - 9781573317146

VL - 1149

T3 - Annals of the New York Academy of Sciences

SP - 312

EP - 314

BT - Animal Biodiversity and Emerging Diseases Prediction and Prevention

PB - Wiley

ER -

Development of specific oligonucleotide probes to detect Vibrio species: A preliminary study

Abstract

Publication series

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this