Development of specific oligonucleotide probes to detect Vibrio species: A preliminary study

Umberto Molini, Narut Thanantong, Annunziata Giangaspero, Olivier A.E. Sparagano

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.

Original languageEnglish
Title of host publicationAnimal Biodiversity and Emerging Diseases Prediction and Prevention
PublisherWiley
Pages312-314
Number of pages3
Volume1149
ISBN (Print)9781573317146
DOIs
Publication statusPublished - 15 Dec 2008
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1149
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Diagnostic
  • PCR
  • Reverse line blot
  • Vibrio

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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  • Cite this

    Molini, U., Thanantong, N., Giangaspero, A., & Sparagano, O. A. E. (2008). Development of specific oligonucleotide probes to detect Vibrio species: A preliminary study. In Animal Biodiversity and Emerging Diseases Prediction and Prevention (Vol. 1149, pp. 312-314). (Annals of the New York Academy of Sciences; Vol. 1149). Wiley. https://doi.org/10.1196/annals.1428.049