TY - CHAP
T1 - Development of specific oligonucleotide probes to detect Vibrio species
T2 - A preliminary study
AU - Molini, Umberto
AU - Thanantong, Narut
AU - Giangaspero, Annunziata
AU - Sparagano, Olivier A.E.
PY - 2008/12/15
Y1 - 2008/12/15
N2 - Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.
AB - Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.
KW - Diagnostic
KW - PCR
KW - Reverse line blot
KW - Vibrio
UR - http://www.scopus.com/inward/record.url?scp=57849098915&partnerID=8YFLogxK
U2 - 10.1196/annals.1428.049
DO - 10.1196/annals.1428.049
M3 - Chapter
C2 - 19120237
AN - SCOPUS:57849098915
SN - 9781573317146
VL - 1149
T3 - Annals of the New York Academy of Sciences
SP - 312
EP - 314
BT - Animal Biodiversity and Emerging Diseases Prediction and Prevention
PB - Wiley
ER -
