Detection of Naegleria fowleri cysts in environmental samples by using a DNA probe

Olivier Sparagano

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In this study we tried to detect DNA Naegleria fowleri in artificially contaminated environmental samples, with or without sediments, containing 104 cysts of this pathogenic amoeba. We used two assays to extract DNA from samples: first, direct DNA extraction, which gave positive results only for water samples without sediment; second, DNA extraction after sample incubation on agar plates, which allowed us to remove amoeba growing out of the sediments, and which gave positive results for all samples, even those initially with sediments (5, 500 or 500 mg). Thus, this molecular identification appears as a powerful tool to investigate N. fowleri growth in environmental samples.

Original languageEnglish
Pages (from-to)349-351
Number of pages3
JournalFEMS Microbiology Letters
Volume112
Issue number3
Publication statusPublished - 15 Sep 1993
Externally publishedYes

Keywords

  • DNA probe
  • Environmental samples
  • Naegleria fowleri
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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