Detection of HHV-5 HHV-6a HHV-6b and HHV-7 in the urine: potential use as a non-invasive diagnostic tool for immune profiling

Shelia Govind; Pierre Olivier Lang; Alexander Bürkle; María Moreno-Villanueva; Claudio Franceschi; Miriam Capri; Jurgen Bernard; Birgit Weinberger; Beatrix Grubeck-Loebenstein; Simone Fiegl; Efstathios S. Gonos; Ewa Sikora; Eugène Jansen; Martijn E. T. Dollé; Tilman Grune; Nicolle Breusing; Richard Aspinall

doi:10.1186/s12979-024-00490-9

Detection of HHV-5 HHV-6a HHV-6b and HHV-7 in the urine: potential use as a non-invasive diagnostic tool for immune profiling

Shelia Govind, Pierre Olivier Lang, Alexander Bürkle, María Moreno-Villanueva, Claudio Franceschi, Miriam Capri, Jurgen Bernard, Birgit Weinberger, Beatrix Grubeck-Loebenstein, Simone Fiegl, Efstathios S. Gonos, Ewa Sikora, Eugène Jansen, Martijn E. T. Dollé, Tilman Grune, Nicolle Breusing, Richard Aspinall

Centre for Intelligent Healthcare

Research output: Contribution to journal › Article › peer-review

9 Downloads (Pure)

Abstract

Decline in immune function with age has been studied extensively, but approaches to immune restoration have been hampered by the lack of simple methods of identifying individuals whose immune system is in decline. Our approach has been to identify individuals whose immune decline has led to a loss of control of common latent viral infections and their consequent reactivation. Viruses excreted in urine were detected and quantified and we believe this approach could provide a 'surrogate marker' for identifying immune compromised individuals. Here we report the detection of human herpes virus (HHV) 5, 6a, 6b and 7 in the urine of healthy individuals over a wide age range and their correlation with T cell receptor excision circle (TREC) data. The results did not show a clear correlation between TREC values and the detection of individual specific viruses or viral load values when measured singly. However, a correlation was found between low TREC values and the detection of several different human herpes viruses in the urine in males. We present evidence suggesting that for males, the detection of three or more different human herpes viruses in the urine could identify individuals with declining immune function as evidenced by their significantly lower TREC levels.

Original language	English
Article number	84
Number of pages	12
Journal	Immunity and Ageing
Volume	21
DOIs	https://doi.org/10.1186/s12979-024-00490-9
Publication status	Published - 29 Nov 2024

Bibliographical note

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material.

Funding

The authors would like to thank the subjects who participated in the MARK-AGE study. All information was accessed in accordance with the applicable laws and ethical requirements for the study period concerned and was compliant with the Declaration of Helsinki. The Local Research Ethics Committees of the respective recruitment centres provided ethical approval for the MARK-AGE project, and this project was registered with the German Clinical Trials Register (DRKS00007713). This work was funded by the European Commission (Project Full Name: European study to Establish Biomarkers of Human Ageing; Project Acronym: MARK-AGE; Project No 200880).

Funders	Funder number
European Commission	200880

Keywords

Human Herpes Virus
T cell receptor excision circles

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1186/s12979-024-00490-9Licence: CC BY

Aspinall2024VORFinal published version, 1.99 MBLicence: CC BY

Cite this

Govind, S., Lang, P. O., Bürkle, A., Moreno-Villanueva, M., Franceschi, C., Capri, M., Bernard, J., Weinberger, B., Grubeck-Loebenstein, B., Fiegl, S., Gonos, E. S., Sikora, E., Jansen, E., Dollé, M. E. T., Grune, T., Breusing, N., & Aspinall, R. (2024). Detection of HHV-5 HHV-6a HHV-6b and HHV-7 in the urine: potential use as a non-invasive diagnostic tool for immune profiling. Immunity and Ageing, 21, Article 84. https://doi.org/10.1186/s12979-024-00490-9

Govind, S, Lang, PO, Bürkle, A, Moreno-Villanueva, M, Franceschi, C, Capri, M, Bernard, J, Weinberger, B, Grubeck-Loebenstein, B, Fiegl, S, Gonos, ES, Sikora, E, Jansen, E, Dollé, MET, Grune, T, Breusing, N & Aspinall, R 2024, 'Detection of HHV-5 HHV-6a HHV-6b and HHV-7 in the urine: potential use as a non-invasive diagnostic tool for immune profiling', Immunity and Ageing, vol. 21, 84. https://doi.org/10.1186/s12979-024-00490-9

@article{c84ab5589dab443aac2778b119c3df42,

title = "Detection of HHV-5 HHV-6a HHV-6b and HHV-7 in the urine: potential use as a non-invasive diagnostic tool for immune profiling",

abstract = "Decline in immune function with age has been studied extensively, but approaches to immune restoration have been hampered by the lack of simple methods of identifying individuals whose immune system is in decline. Our approach has been to identify individuals whose immune decline has led to a loss of control of common latent viral infections and their consequent reactivation. Viruses excreted in urine were detected and quantified and we believe this approach could provide a 'surrogate marker' for identifying immune compromised individuals. Here we report the detection of human herpes virus (HHV) 5, 6a, 6b and 7 in the urine of healthy individuals over a wide age range and their correlation with T cell receptor excision circle (TREC) data. The results did not show a clear correlation between TREC values and the detection of individual specific viruses or viral load values when measured singly. However, a correlation was found between low TREC values and the detection of several different human herpes viruses in the urine in males. We present evidence suggesting that for males, the detection of three or more different human herpes viruses in the urine could identify individuals with declining immune function as evidenced by their significantly lower TREC levels.",

keywords = "Human Herpes Virus, T cell receptor excision circles",

author = "Shelia Govind and Lang, {Pierre Olivier} and Alexander B{\"u}rkle and Mar{\'i}a Moreno-Villanueva and Claudio Franceschi and Miriam Capri and Jurgen Bernard and Birgit Weinberger and Beatrix Grubeck-Loebenstein and Simone Fiegl and Gonos, {Efstathios S.} and Ewa Sikora and Eug{\`e}ne Jansen and Doll{\'e}, {Martijn E. T.} and Tilman Grune and Nicolle Breusing and Richard Aspinall",

note = "Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article{\textquoteright}s Creative Commons licence, unless indicated otherwise in a credit line to the material.",

year = "2024",

month = nov,

day = "29",

doi = "10.1186/s12979-024-00490-9",

language = "English",

volume = "21",

journal = "Immunity and Ageing",

issn = "1742-4933",

publisher = "Springer Verlag",

}

TY - JOUR

T1 - Detection of HHV-5 HHV-6a HHV-6b and HHV-7 in the urine

T2 - potential use as a non-invasive diagnostic tool for immune profiling

AU - Govind, Shelia

AU - Lang, Pierre Olivier

AU - Bürkle, Alexander

AU - Moreno-Villanueva, María

AU - Franceschi, Claudio

AU - Capri, Miriam

AU - Bernard, Jurgen

AU - Weinberger, Birgit

AU - Grubeck-Loebenstein, Beatrix

AU - Fiegl, Simone

AU - Gonos, Efstathios S.

AU - Sikora, Ewa

AU - Jansen, Eugène

AU - Dollé, Martijn E. T.

AU - Grune, Tilman

AU - Breusing, Nicolle

AU - Aspinall, Richard

N1 - Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material.

PY - 2024/11/29

Y1 - 2024/11/29

N2 - Decline in immune function with age has been studied extensively, but approaches to immune restoration have been hampered by the lack of simple methods of identifying individuals whose immune system is in decline. Our approach has been to identify individuals whose immune decline has led to a loss of control of common latent viral infections and their consequent reactivation. Viruses excreted in urine were detected and quantified and we believe this approach could provide a 'surrogate marker' for identifying immune compromised individuals. Here we report the detection of human herpes virus (HHV) 5, 6a, 6b and 7 in the urine of healthy individuals over a wide age range and their correlation with T cell receptor excision circle (TREC) data. The results did not show a clear correlation between TREC values and the detection of individual specific viruses or viral load values when measured singly. However, a correlation was found between low TREC values and the detection of several different human herpes viruses in the urine in males. We present evidence suggesting that for males, the detection of three or more different human herpes viruses in the urine could identify individuals with declining immune function as evidenced by their significantly lower TREC levels.

AB - Decline in immune function with age has been studied extensively, but approaches to immune restoration have been hampered by the lack of simple methods of identifying individuals whose immune system is in decline. Our approach has been to identify individuals whose immune decline has led to a loss of control of common latent viral infections and their consequent reactivation. Viruses excreted in urine were detected and quantified and we believe this approach could provide a 'surrogate marker' for identifying immune compromised individuals. Here we report the detection of human herpes virus (HHV) 5, 6a, 6b and 7 in the urine of healthy individuals over a wide age range and their correlation with T cell receptor excision circle (TREC) data. The results did not show a clear correlation between TREC values and the detection of individual specific viruses or viral load values when measured singly. However, a correlation was found between low TREC values and the detection of several different human herpes viruses in the urine in males. We present evidence suggesting that for males, the detection of three or more different human herpes viruses in the urine could identify individuals with declining immune function as evidenced by their significantly lower TREC levels.

KW - Human Herpes Virus

KW - T cell receptor excision circles

UR - http://www.scopus.com/inward/record.url?scp=85211120139&partnerID=8YFLogxK

U2 - 10.1186/s12979-024-00490-9

DO - 10.1186/s12979-024-00490-9

M3 - Article

SN - 1742-4933

VL - 21

JO - Immunity and Ageing

JF - Immunity and Ageing

M1 - 84

ER -

Detection of HHV-5 HHV-6a HHV-6b and HHV-7 in the urine: potential use as a non-invasive diagnostic tool for immune profiling

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this