Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily

K. Georges; G. R. Loria; S. Riili; A. Greco; S. Caracappa; F. Jongejan; O. Sparagano

doi:10.1016/S0304-4017(01)00488-5

Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily

K. Georges, G. R. Loria, S. Riili, A. Greco, S. Caracappa, F. Jongejan, O. Sparagano

Research output: Contribution to journal › Article › peer-review

218 Citations (Scopus)

Abstract

A reverse line blot hybridisation (RLB) of 21 oligonucleotides with polymerase chain reaction (PCR) amplified regions of 16S rRNA (Ehrlichia/Anaplasma group) or 18S rRNA (Babesia/Theileria group) genes of haemoparasites detected Theileria annulata, T. buffeli/orientalis, Babesia bovis, B. bigemina, B. divergens, Ehrlichia bovis, Anaplasma marginale, A. centrale and unknown species within the Rickettsia tribe. A very high prevalence of mixed infections was detected, which indicated that animals infected with Babesia spp. were also infected with Theileria spp. and/or Anaplasma spp. The tick distribution appeared to be seasonal with Hyalomma marginatum as the most frequently observed tick and Boophilus annulatus and Ixodes ricinus as the least frequently observed ticks. Other species identified in the 818 ticks collected during the five sampling periods between April 1998 and November 1999 included H. lusitanicum, Rhipicephalus sanguineus group, R. bursa, Dermacentor marginatus, Haemaphysalis punctata, B. annulatus and I. ricinus.

Original language	English
Pages (from-to)	273-286
Number of pages	14
Journal	Veterinary Parasitology
Volume	99
Issue number	4
DOIs	https://doi.org/10.1016/S0304-4017(01)00488-5
Publication status	Published - 31 Aug 2001
Externally published	Yes

Funding

The authors would like to thank the veterinarians, Dr. C. Sammartino, Dr. G. Tumino, Dr. M. Vicari, Dr. G. Cassata, Dr. N. Galati and Dr. M. Fiasconaro who assisted in the field collections and the farmers who agreed to participate in the survey. Fieldwork has been done thanks to funding from NUFFIC for K.G. and the EU Marie Curie Fellowship ERBFCIBT 9832000 for O.S. and visit grants paid by I.Z.S. to K.G. and O.S. The authors would like to thank Dr. Alan Younger from the Department of Agriculture at the University of Newcastle for correcting this paper. We are also thankful to the referees who helped us to improve this manuscript.

Keywords

Cattle
Epidemiology
PCR
Reverse line blot
Sicily
Tick-borne diseases
Ticks

ASJC Scopus subject areas

Animal Science and Zoology
Parasitology
General Veterinary

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10.1016/S0304-4017(01)00488-5

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title = "Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily",

abstract = "A reverse line blot hybridisation (RLB) of 21 oligonucleotides with polymerase chain reaction (PCR) amplified regions of 16S rRNA (Ehrlichia/Anaplasma group) or 18S rRNA (Babesia/Theileria group) genes of haemoparasites detected Theileria annulata, T. buffeli/orientalis, Babesia bovis, B. bigemina, B. divergens, Ehrlichia bovis, Anaplasma marginale, A. centrale and unknown species within the Rickettsia tribe. A very high prevalence of mixed infections was detected, which indicated that animals infected with Babesia spp. were also infected with Theileria spp. and/or Anaplasma spp. The tick distribution appeared to be seasonal with Hyalomma marginatum as the most frequently observed tick and Boophilus annulatus and Ixodes ricinus as the least frequently observed ticks. Other species identified in the 818 ticks collected during the five sampling periods between April 1998 and November 1999 included H. lusitanicum, Rhipicephalus sanguineus group, R. bursa, Dermacentor marginatus, Haemaphysalis punctata, B. annulatus and I. ricinus.",

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AU - Georges, K.

AU - Loria, G. R.

AU - Riili, S.

AU - Greco, A.

AU - Caracappa, S.

AU - Jongejan, F.

AU - Sparagano, O.

PY - 2001/8/31

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N2 - A reverse line blot hybridisation (RLB) of 21 oligonucleotides with polymerase chain reaction (PCR) amplified regions of 16S rRNA (Ehrlichia/Anaplasma group) or 18S rRNA (Babesia/Theileria group) genes of haemoparasites detected Theileria annulata, T. buffeli/orientalis, Babesia bovis, B. bigemina, B. divergens, Ehrlichia bovis, Anaplasma marginale, A. centrale and unknown species within the Rickettsia tribe. A very high prevalence of mixed infections was detected, which indicated that animals infected with Babesia spp. were also infected with Theileria spp. and/or Anaplasma spp. The tick distribution appeared to be seasonal with Hyalomma marginatum as the most frequently observed tick and Boophilus annulatus and Ixodes ricinus as the least frequently observed ticks. Other species identified in the 818 ticks collected during the five sampling periods between April 1998 and November 1999 included H. lusitanicum, Rhipicephalus sanguineus group, R. bursa, Dermacentor marginatus, Haemaphysalis punctata, B. annulatus and I. ricinus.

AB - A reverse line blot hybridisation (RLB) of 21 oligonucleotides with polymerase chain reaction (PCR) amplified regions of 16S rRNA (Ehrlichia/Anaplasma group) or 18S rRNA (Babesia/Theileria group) genes of haemoparasites detected Theileria annulata, T. buffeli/orientalis, Babesia bovis, B. bigemina, B. divergens, Ehrlichia bovis, Anaplasma marginale, A. centrale and unknown species within the Rickettsia tribe. A very high prevalence of mixed infections was detected, which indicated that animals infected with Babesia spp. were also infected with Theileria spp. and/or Anaplasma spp. The tick distribution appeared to be seasonal with Hyalomma marginatum as the most frequently observed tick and Boophilus annulatus and Ixodes ricinus as the least frequently observed ticks. Other species identified in the 818 ticks collected during the five sampling periods between April 1998 and November 1999 included H. lusitanicum, Rhipicephalus sanguineus group, R. bursa, Dermacentor marginatus, Haemaphysalis punctata, B. annulatus and I. ricinus.

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KW - Epidemiology

KW - PCR

KW - Reverse line blot

KW - Sicily

KW - Tick-borne diseases

KW - Ticks

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Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily

Abstract

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Keywords

ASJC Scopus subject areas

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