Design and development of novel mitochondrial targeted nanocarriers, DQAsomes for curcumin inhalation

Špela Zupančič, Petra Kocbek, M Gulrez Zariwala, Derek Renshaw, Mine Orlu Gul, Zeeneh Elsaid, Kevin M G Taylor, Satyanarayana Somavarapu

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Curcumin has potent antioxidant and anti-inflammatory properties but poor absorption following oral administration owing to its low aqueous solubility. Development of novel formulations to improve its in vivo efficacy is therefore challenging. In this study, formulation of curcumin-loaded DQAsomes (vesicles formed from the amphiphile, dequalinium) for pulmonary delivery is presented for the first time. The vesicles demonstrated mean hydrodynamic diameters between 170 and 200 nm, with a ζ potential of approximately +50 mV, high drug loading (up to 61%) and encapsulation efficiency (90%), resulting in enhanced curcumin aqueous solubility. Curcumin encapsulation in DQAsomes in the amorphous state was confirmed by X-ray diffraction and differential scanning calorimetry analysis. The existence of hydrogen bonds and cation-π interaction between curcumin and vesicle building blocks, namely dequalinium molecules, were shown in lyophilized DQAsomes using FT-IR analysis. Encapsulation of curcumin in DQAsomes enhanced the antioxidant activity of curcumin compared to free curcumin. DQAsome dispersion was successfully nebulized with the majority of the delivered dose deposited in the second stage of the twin-stage impinger. The vesicles showed potential for mitochondrial targeting. Curcumin-loaded DQAsomes thus represent a promising inhalation formulation with improved stability characteristics and mitochondrial targeting ability, indicating a novel approach for efficient curcumin delivery for effective treatment of acute lung injury and the rationale for future in vivo studies.

Original languageEnglish
Pages (from-to)2334-2345
Number of pages12
JournalMolecular Pharmaceutics
Volume11
Issue number7
Early online date22 May 2014
DOIs
Publication statusPublished - 7 Jul 2014

Keywords

  • Administration, Inhalation
  • Chemistry, Pharmaceutical
  • Curcumin
  • Dequalinium
  • Drug Carriers
  • Drug Delivery Systems
  • Mitochondria
  • Nanoparticles
  • Particle Size
  • Solubility
  • X-Ray Diffraction
  • Journal Article

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    Zupančič, Š., Kocbek, P., Zariwala, M. G., Renshaw, D., Gul, M. O., Elsaid, Z., ... Somavarapu, S. (2014). Design and development of novel mitochondrial targeted nanocarriers, DQAsomes for curcumin inhalation. Molecular Pharmaceutics, 11(7), 2334-2345. https://doi.org/10.1021/mp500003q