TY - JOUR
T1 - Cytogenotoxic evaluation of the acetonitrile extract, citrinin and dicitrinin-A from Penicillium citrinum
AU - de Oliveira Filho, José Williams Gomes
AU - Andrade, Teresinha de Jesus Aguiar Dos Santos
AU - de Lima, Rosália Maria Tôrres
AU - Silva, Dulce Helena Siqueira
AU - Dos Reis, Antonielly Campinho
AU - Santos, José Victor de Oliveira
AU - de Meneses, Ag-Anne Pereira Melo
AU - de Carvalho, Ricardo Melo
AU - da Mata, Ana Maria Oliveira
AU - de Alencar, Marcus Vinícius Oliveira Barros
AU - Dias, Ana Carolina Soares
AU - da Silva, Felipe Cavalcanti Carneiro
AU - Islam, Muhammad Torequl
AU - Clark, Cain C T
AU - Sousa, João Marcelo de Castro E
AU - Melo-Cavalcante, Ana Amélia de Carvalho
PY - 2020/5/25
Y1 - 2020/5/25
N2 - Endophytic fungi are promising sources of bioactive substances; however, their secondary metabolites are toxic to plants, animals, and humans. This study aimed toevaluate the toxic, cytotoxic, mutagenic and oxidant/antioxidant activities of acetonitrile extract (AEPc), citrinin (CIT) and dicitrinin-A (DIC-A) of Penicillium citrinum. For this, the test substances at 0.5; 1.0; 1.5 and 2 μg/mLwere exposed for 24 and 48 h in Artemia salina, and 48 h in Allium cepa test systems. The oxidant/antioxidant test was evaluated in pre-, co- and post-treatment with the stressor hydrogen peroxide (H2O2) in Saccharomyces cerevisiae. The results suggest that the AEPc, CIT and DIC-A at 0.5; 1.0; 1.5 and 2 μg/mL showed toxicity in A. saline, with LC50 (24 h) of 2.03 μg/mL, 1.71 μg/mL and 2.29 μg/mL, and LC50 (48 h) of 0.51 μg/mL, 0.54 μg/mL and 0.54 μg/mL, respectively.In A. cepa, the test substances also exerted cytotoxic and mutagenic effects. The AEPc, CIT and DIC-A at lower concentrations modulated the damage induced by H2O2 in the proficient and mutant strains of S. cerevisiae for cytoplasmic and mitochondrial superoxide dismutase. Moreover, the AEPc at 2 μg/mL and CIT at the two highest concentrations did not affect the H2O2-induced DNA damage in the test strains. In conclusion, AEPc, CIT and DIC-A of P. citrinum may exert their toxic, cytotoxic and mutagenic effects in the test systems possibly through oxidative stress induction pathway.
AB - Endophytic fungi are promising sources of bioactive substances; however, their secondary metabolites are toxic to plants, animals, and humans. This study aimed toevaluate the toxic, cytotoxic, mutagenic and oxidant/antioxidant activities of acetonitrile extract (AEPc), citrinin (CIT) and dicitrinin-A (DIC-A) of Penicillium citrinum. For this, the test substances at 0.5; 1.0; 1.5 and 2 μg/mLwere exposed for 24 and 48 h in Artemia salina, and 48 h in Allium cepa test systems. The oxidant/antioxidant test was evaluated in pre-, co- and post-treatment with the stressor hydrogen peroxide (H2O2) in Saccharomyces cerevisiae. The results suggest that the AEPc, CIT and DIC-A at 0.5; 1.0; 1.5 and 2 μg/mL showed toxicity in A. saline, with LC50 (24 h) of 2.03 μg/mL, 1.71 μg/mL and 2.29 μg/mL, and LC50 (48 h) of 0.51 μg/mL, 0.54 μg/mL and 0.54 μg/mL, respectively.In A. cepa, the test substances also exerted cytotoxic and mutagenic effects. The AEPc, CIT and DIC-A at lower concentrations modulated the damage induced by H2O2 in the proficient and mutant strains of S. cerevisiae for cytoplasmic and mitochondrial superoxide dismutase. Moreover, the AEPc at 2 μg/mL and CIT at the two highest concentrations did not affect the H2O2-induced DNA damage in the test strains. In conclusion, AEPc, CIT and DIC-A of P. citrinum may exert their toxic, cytotoxic and mutagenic effects in the test systems possibly through oxidative stress induction pathway.
KW - Endophytic fungi
KW - Penicillium citrinum
KW - cytotoxicity
KW - genotoxicity
KW - mutagenicity
KW - mycotoxins
UR - http://www.scopus.com/inward/record.url?scp=85086038545&partnerID=8YFLogxK
U2 - 10.1080/01480545.2020.1769642
DO - 10.1080/01480545.2020.1769642
M3 - Article
C2 - 32448000
VL - (In-Press)
SP - (In-Press)
JO - Drug and Chemical Toxicology
JF - Drug and Chemical Toxicology
SN - 0148-0545
ER -