Critical values of the volumetric oxygen transfer coefficient and oxygen concentration that prevent oxygen limitation in ferrous iron and elemental sulfur oxidation by Acidithiobacillus ferrooxidans

Martin Mandl; Eva Pakostova; Lenka Poskerova

doi:10.1016/j.hydromet.2014.09.009

Critical values of the volumetric oxygen transfer coefficient and oxygen concentration that prevent oxygen limitation in ferrous iron and elemental sulfur oxidation by Acidithiobacillus ferrooxidans

Martin Mandl, Eva Pakostova, Lenka Poskerova

Masaryk University

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

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Abstract

Biochemical and biotechnological aspects of aeration conditions in iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans cultures were studied. The critical values of volumetric oxygen transfer coefficient, (kLa)crit, and oxygen concentration, Ccrit, were used to define the conditions necessary for minimum aeration and to eliminate potential oxygen limitation during substrate oxidation by themodel bioleaching culture. TheMichaelis constants for oxygenwere 1.07 and 0.61 μmol O2 l−1 for the oxidation of ferrous iron and elemental sulfur, respectively. The Ccrit values, below which oxygen limitation may occur, were 6.250 and 3.125 μmol O2 l−1, determined in respirometric experiments for the oxidation of ferrous iron and elemental sulfur, respectively. The rates of iron and sulfur oxidation were also determined at the Ccrit values by measuring the formation of ferric iron and protons in the corresponding cell suspensions. Neither iron nor sulfur oxidation was limited by oxygen at Ccrit. The (kLa)crit values required to maintain oxygen-unlimited oxidation of ferrous iron and elemental sulfur were 7.70 and 4.88 h−1, respectively.

Original language	English
Pages (from-to)	276-280
Number of pages	5
Journal	Hydrometallurgy
Volume	150
Early online date	28 Sept 2014
DOIs	https://doi.org/10.1016/j.hydromet.2014.09.009
Publication status	Published - Dec 2014
Externally published	Yes

Bibliographical note

NOTICE: this is the author’s version of a work that was accepted for publication in Hydrometallurgy. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Hydrometallurgy, [150, (2015)] DOI: 10.1016/j.hydromet.2014.09.009

© 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

Acidithiobacillus ferrooxidans
Aeration
Oxygen limitation
Volumetric oxygen transfer coefficient

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10.1016/j.hydromet.2014.09.009

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Critical values of the volumetric oxygen transfer coefficient and oxygen concentration that prevent oxygen limitation in ferrous iron and elemental sulfur oxidation by Acidithiobacillus ferrooxidans. / Mandl, Martin; Pakostova, Eva; Poskerova, Lenka.
In: Hydrometallurgy, Vol. 150, 12.2014, p. 276-280.

Research output: Contribution to journal › Article › peer-review

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title = "Critical values of the volumetric oxygen transfer coefficient and oxygen concentration that prevent oxygen limitation in ferrous iron and elemental sulfur oxidation by Acidithiobacillus ferrooxidans",

abstract = "Biochemical and biotechnological aspects of aeration conditions in iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans cultures were studied. The critical values of volumetric oxygen transfer coefficient, (kLa)crit, and oxygen concentration, Ccrit, were used to define the conditions necessary for minimum aeration and to eliminate potential oxygen limitation during substrate oxidation by themodel bioleaching culture. TheMichaelis constants for oxygenwere 1.07 and 0.61 μmol O2 l−1 for the oxidation of ferrous iron and elemental sulfur, respectively. The Ccrit values, below which oxygen limitation may occur, were 6.250 and 3.125 μmol O2 l−1, determined in respirometric experiments for the oxidation of ferrous iron and elemental sulfur, respectively. The rates of iron and sulfur oxidation were also determined at the Ccrit values by measuring the formation of ferric iron and protons in the corresponding cell suspensions. Neither iron nor sulfur oxidation was limited by oxygen at Ccrit. The (kLa)crit values required to maintain oxygen-unlimited oxidation of ferrous iron and elemental sulfur were 7.70 and 4.88 h−1, respectively.",

keywords = "Acidithiobacillus ferrooxidans, Aeration, Oxygen limitation, Volumetric oxygen transfer coefficient",

author = "Martin Mandl and Eva Pakostova and Lenka Poskerova",

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AU - Poskerova, Lenka

N1 - NOTICE: this is the author’s version of a work that was accepted for publication in Hydrometallurgy. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Hydrometallurgy, [150, (2015)] DOI: 10.1016/j.hydromet.2014.09.009 © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

PY - 2014/12

Y1 - 2014/12

N2 - Biochemical and biotechnological aspects of aeration conditions in iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans cultures were studied. The critical values of volumetric oxygen transfer coefficient, (kLa)crit, and oxygen concentration, Ccrit, were used to define the conditions necessary for minimum aeration and to eliminate potential oxygen limitation during substrate oxidation by themodel bioleaching culture. TheMichaelis constants for oxygenwere 1.07 and 0.61 μmol O2 l−1 for the oxidation of ferrous iron and elemental sulfur, respectively. The Ccrit values, below which oxygen limitation may occur, were 6.250 and 3.125 μmol O2 l−1, determined in respirometric experiments for the oxidation of ferrous iron and elemental sulfur, respectively. The rates of iron and sulfur oxidation were also determined at the Ccrit values by measuring the formation of ferric iron and protons in the corresponding cell suspensions. Neither iron nor sulfur oxidation was limited by oxygen at Ccrit. The (kLa)crit values required to maintain oxygen-unlimited oxidation of ferrous iron and elemental sulfur were 7.70 and 4.88 h−1, respectively.

AB - Biochemical and biotechnological aspects of aeration conditions in iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans cultures were studied. The critical values of volumetric oxygen transfer coefficient, (kLa)crit, and oxygen concentration, Ccrit, were used to define the conditions necessary for minimum aeration and to eliminate potential oxygen limitation during substrate oxidation by themodel bioleaching culture. TheMichaelis constants for oxygenwere 1.07 and 0.61 μmol O2 l−1 for the oxidation of ferrous iron and elemental sulfur, respectively. The Ccrit values, below which oxygen limitation may occur, were 6.250 and 3.125 μmol O2 l−1, determined in respirometric experiments for the oxidation of ferrous iron and elemental sulfur, respectively. The rates of iron and sulfur oxidation were also determined at the Ccrit values by measuring the formation of ferric iron and protons in the corresponding cell suspensions. Neither iron nor sulfur oxidation was limited by oxygen at Ccrit. The (kLa)crit values required to maintain oxygen-unlimited oxidation of ferrous iron and elemental sulfur were 7.70 and 4.88 h−1, respectively.

KW - Acidithiobacillus ferrooxidans

KW - Aeration

KW - Oxygen limitation

KW - Volumetric oxygen transfer coefficient

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DO - 10.1016/j.hydromet.2014.09.009

M3 - Article

SN - 0304-386X

VL - 150

SP - 276

EP - 280

JO - Hydrometallurgy

JF - Hydrometallurgy

ER -

Critical values of the volumetric oxygen transfer coefficient and oxygen concentration that prevent oxygen limitation in ferrous iron and elemental sulfur oxidation by Acidithiobacillus ferrooxidans

Abstract

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Keywords

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